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Study on earlier pathological changes after injury of in vitro cultured retinal Müller cell
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- Author:
- No author available
- Journal Title:
- INTERNATIONAL EYE SCIENCE
- Issue:
- 8
- DOI:
- 10.3969/j.issn.1672-5123.2012.08.08
- Key Word:
- Müller细胞;细胞培养;体外创伤模型;胶质纤维酸性蛋白;细胞凋亡
Abstract： 目的:研究体外培养Müller细胞致伤后早期细胞发生的一系列损伤性变化和发生反应性胶质化的规律.方法:新生SD大鼠视网膜进行体外Müller细胞的培养并建立高压气体冲击损伤模型,检测细胞致伤后早期胶质纤维酸性蛋白(GFAP)表达变化,以及乳酸脱氢酶(LDH)的释放量和细胞凋亡率的变化.结果:致伤后3h左右GFAP的表达量明显增强,以后24h内持续性高表达,并与损伤强度呈正比(P<0.05);LDH的释放增加主要出现在损伤后30min内;损伤后30min各组致伤细胞凋亡率呈非显著性差异(P>0.05),3h后细胞凋亡率增高,6h后细胞凋亡率最高.结论:Müller细胞形态的改变和细胞膜通透性的改变可能是触发GFAP 表达增强的因素之一.
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