Anti cataract potential of phyllanthus niruri in galactose induced cataractogenesis of rat
- Author:
- No author available
- Journal Title:
- INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
- Issue:
- 6
- DOI:
- 10.3969/j.issn.1672-5123.2009.06.001
- Key Word:
- 半乳糖性白内障;谷胱甘肽;山梨糖醇;珠子草;galactose cataract;glutathione;polyols;phyllanthus niruri
Abstract： AIM: To evaluate anti cataract effect of phyllanthus niruri (PN) both in vitro and in vivo galactose induced cataract.METHODS: Aqueous extract of PN was evaluated against galactose-induced cataract both in vitro and in vivo. Galactosaemic cataract was induced in rats by feeding 300g/L galactose diet. PN was administered orally at three-dose levels 75, 150 and 300 mg/kg of body weight. Rat lenses were subjected to osmotic stress in vitro by incorporating galactose (30mmol/L) in the culture medium. The effect of PN (720 and 880μg/mL) on the glutathione (GSH) and polyols levels was studied.RESULTS: PN significantly delayed the onset and progression of cataract in vivo. In addition to the delay in reaching various stages of development of cataract, stage IV did not develop with lower doses till the completion of experimental period. Lenses treated with PN 880μg/mL concentration showed higher levels of GSH and decreased levels of polyols in vitro. In vivo, 75mg/kg significantly delayed the onset and progression of cataract as compared to control.CONCLUSION: PN delayed the process of cataracto-genesis in the experimental models. However, further study is required to extrapolate the use in human beings for the prevention of cataract.
- [1]S N, Ughade,S P, Zodpey,V A, Khanolkar.Risk factors for cataract: a case control study.[J].1998,46(4).
- [2]A Y, Lee,S S, Chung.Contributions of polyol pathway to oxidative stress in diabetic cataract.[J].1999,13(1).
- [3]Group of Indian Journal of Pharmacology.Effect of Pongamia pinnata flowers on blood glucose and oxidative stress in alloxan induced diabetic rats[J].2006,38(1).
- [4]Grover J,Yadav S,Dhunnoo Y,etc.Hypoglycemic and antihyperglycemic activity of Murraya koenigii leaves in diabetic rats.[J].2002,82(2).
- [5]Sethi S,Bedi KL,Singh J,etc.Hepatocurative and antioxidant profile of HP-1, a polyherbal phytomedicine.[J].2003,22(12).
- [6]M, Wang,H, Cheng,Y, Li,etc.Herbs of the genus Phyllanthus in the treatment of chronic hepatitis B: observations with three preparations from different geographic sites.[J].1995,126.
- [7]R, Mehrotra,S, Rawat,D K, Kulshreshtha,etc.In vitro studies on the effect of certain natural products against hepatitis B virus.[J].1990,92.
- [8]Spector A.Oxidative stress-induced cataract: mechanism of action.[J].1995,9(12).
- [9]R. C. Girme,P. S. Patki,D. G. Langade,etc.In vitro prevention by ACE Inhibitors of cataract induced by glucose[J].2006,38(2).
- [10]M S, Moron,J W, Depierre,B, Mannervik.Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver.[J].1979,582.
- [11]Ayala MN,Soderberg PG.Vitamin E can protect against ultraviolet radiation-induced cataract in albino rats.[J].2004,36(5).
- [12]Bauer V,Kyselova Z,Stefek M.Pharmacological prevention of diabetic cataract.[J].2004,18(2).
- [13]V N, Reddy,R, Garadi,B, Chakrapani,etc.Effect of glutathione depletion on cation transport and metabolism in the rabbit lens.[J].1988,20.
- [14]Krishnaswamy K,Suryanarayana P,Krishna TP,etc.Curcumin and turmeric delay streptozotocin-induced diabetic cataract in rats.[J].2005,46(6).
- [15]Thylefors, B..Avoidable blindness.[J].1999,77(6).
- [16]J H, Kinoshita.A thirty year journey in the polyol pathway.[J].1990,50(6).
- [17]N, Srividya,S, Periwal.Diuretic, hypotensive and hypoglycaemic effect of Phyllanthus amarus.[J].1995,33.
- [18]T O, Sippel.Changes in the water, protein, and glutathione contents of the lens in the course of galactose cataract development in rats.[J].1966,5(6).
- [19].Formation of Polyols by the Lens of the Rat withSugarCataract[J].1959,184(4681).
- [20]Srivastava S,Joshi S,Gupta SK,etc.Green Tea (Camellia sinensis) Protects against Selenite-Induced Oxidative Stress in Experimental Cataractogenesis.[J].2002,34(4).
- [21]Harding J. J..Free and protein-bound glutathione in normal and cataractous human lenses[J].1970.
- [22]J H, Kinoshita,P, Kador,M, Catiles.Aldose reductase in diabetic cataracts.[J].1981,246.
- [23]Ismail MF,Gad MZ,El-Sawalhi MM,etc.Biochemical study of the anti-diabetic action of the Egyptian plants Fenugreek and Balanites[J].2006,281(1).