Abstract： Objective To investigate the effects of growth arrest-specific protein 6 on blood coagulation in septic mice.Methods Septic mice model was induced by cecal ligation and puncture (CLP).One hundred and twenty male BALB/c mice of clean grade were randomly (random number) divided into six groups : sham operation group (n =20), CLP group (n =20), CLP + vehicle group (n =20), CLP+Gas6 (Growth Arrest-Specific Protein 6) (1 μg) group (n=20), CLP+Gas6 (3 μg) group (n =20) and CLP + Gas6 (6 μg) group (n =20).All the survived mice were sacrificed after 24 h of operation.Plasma prothrombin time (PT), thrombin time (TT), active partial thrombo-plastin time (APTT), fibrinogen (Fbg), d-dimer (DD) and Platelets (PLT) count was measured.Plasma tissue factor (TF), P-selectin, Intercellular Adhesion Molecule 1 (ICAM-1) was measured by ELISA method.Statistical analyses were performed using one-way analysis of variance (SPSS18.0, Chicago, USA) test followed by Bonferroni test to compare three or more groups or by Student' s t-test to compare two groups.Results Plasma PT, TT, APTT, DD, TF, P-selectin and ICAM-1 in CLP group was longer or higher than that in sham group (P =0.000), while Fbg and PLT in CLP + vehicle group were lower than that in sham group (P =0.000).Treatment with rmGas6 significantly increase plasma PT, TT, APTT, Fbg, DD, PLT, TF, P-selectin and ICAM-1 in septic mice (P ＜ 0.01).Conclusions Gas6 can activate coagulation system and aggravate coagulation dysfunction in septic mices, which may partially through activate endothelial cells and up-regulate TF levels.