Clinical significance of serum miR-888-5p in patients with primary hepatocellular carcinoma
- Author:
- No author available
- Journal Title:
- World Chinese Journal of Digestology
- Issue:
- 7
- DOI:
- 10.11569/wcjd.v28.i7.247
- Key Word:
- miRNA;原发性肝细胞癌;诊断;临床特征;预后
Abstract： 背景miR-888-5p高表达于原发性肝细胞癌(hepatocellular carcinoma,HCC)组织及-HCC细胞系,可促进肿瘤细胞侵袭及转移,与疾病分期及预后不良相关.然而HCC患者血清中miR-888-5p的表达水平尚未被检测,血清miR-888-5p对于HCC诊断及预后判断相关价值尚未被评估.目的检测HCC患者血清中miR-888-5p表达水平,探究其对HCC的诊断价值及与临床特征之间的关系.方法收集68例HCC患者、46例慢性乙型病毒性肝炎(chronic hepatitis B,CHB)患者、43例肝硬化(liver cirrhosis,LC)患者,及40例同期健康体检者,实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction,qRT-PCR)法检测血清中miR-888-5p表达量,受试者工作特征(receiver operating characteristic,ROC)曲线评估其诊断HCC的价值,并分析与HCC临床特征之间的关系.结果HCC患者血清中miR-888-5p表达量较CHB、LC及健康者显著上调(P＜0.05).ROC曲线提示,联合检测ROC曲线下面积(area under curve,AUC)为0.907,敏感性91.18％,特异性72.50％)对HCC诊断价值优于分别检测血清miR-888-5p (AUC=0.737,敏感性79.41％,特异性62.50％)及甲胎蛋白(alpha-fetoprotein,AFP)(AUC =0.819,敏感性73.53％,特异性97.50％),且对AFP阴性HCC也具有较高的诊断价值:AUC=0.793,敏感性90.90％,特异性62.50％.此外,miR-888-5p表达水平与HCC肺转移相关(P=0.01).结论血清miR-888-5p对HCC及AFP阴性HCC具有较高诊断价值,与AFP联合检测诊断价值更高.miR-888-5p表达水平与HCC肺转移相关,有望成为HCC早期诊断及评估预后的新型血清学分子标志物.
- [1]黄湘俊,张文兴.肝细胞癌中microRNA-888基因家族的表达特征及临床意义[J].2019,(1).
- [2]Wanqing CHEN,Rongshou ZHENG,Siwei ZHANG,etc.Cancer incidence and mortality in China in 2013: an analysis based on urbanization level[J].2017,(1).
- [3]Xi Chen,Xing Guo,Qibin Li,etc.Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases[J].2008,(10).
- [4]Lewis,H.,Lance,R.,Troyer,D.,etc.MiR-888 is an expressed prostatic secretionsderived microRNA that promotes prostate cell growth and migration[J].2014,13(2).
- [5]Y-B,Li,F-N,Sun,X-Y,Ma,etc.MiR-888 promotes cell migration and invasion of hepatocellular carcinoma by targeting SMAD4.[J].2019,23(5).
- [6]Margaret S, Ebert,Phillip A, Sharp.Roles for microRNAs in conferring robustness to biological processes.[J].2012,149(3).
- [7]Su-Jun, Gao,Lei, Chen,Wei, Lu,etc.miR-888 functions as an oncogene and predicts poor prognosis in colorectal cancer.[J].2018,15(6).
- [8]Laura,Kulik,Hashem B,El-Serag.Epidemiology and Management of Hepatocellular Carcinoma.[J].2018.
- [9]H Katayama,J Qi,J Wang,etc.Circulating microRNAs (cmiRNAs) as novel potential biomarkers for hepatocellular carcinoma.[J].2013,60(2).
- [10]Livak KJ,Schmittgen TD.Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.[J].2001,25(4).
- [11]Kun-Lin, Xie,Yan-Ge, Zhang,Jun, Liu,etc.MicroRNAs associated with HBV infection and HBV-related HCC.[J].2014,4.
- [12]David P. Bartel.MicroRNAs: Target Recognition and Regulatory Functions[J].2009,136(2).
- [13]De-Cai, Yu,Qing-Guo, Li,Xi-Wei, Ding,etc.Circulating MicroRNAs: Potential Biomarkers for Cancer.[J].2011,12(3).
- [14]Ying, Zhang,Tao, Li,Yumin, Qiu,etc.Serum microRNA panel for early diagnosis of the onset of hepatocellular carcinoma.[J].2017,96(2).
- [15]Filella, Xavier,Foj, Laura.miRNAs as novel biomarkers in the management of prostate cancer[J].2017,55(5).
- [16]Yasuda T,Saigo K,Shimotohno K,etc.Comprehensive analysis of microRNA expression patterns in hepatocellular carcinoma and non-tumorous tissues.[J].2006,25(17).
- [17]Huang,S.,Chen,L..MiR-888 regulates side population properties and cancer metastasis in breast cancer cells[J].2014,450(4).
- [18]E, Hao,J, Yu,S, Xie,etc.Up-regulation of miR-888-5p in hepatocellular carcinoma cell lines and its effect on malignant characteristics of cells.[J].1900,31.
- [19]Fritz, BR,Gentleman, R,Knudsen, BS,etc.Circulating microRNAs as stable blood-based markers for cancer detection[J].2008,105(30).