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Downregulation of miRNA-214 inhibits cisplatin resistance, cell migration, and epithelial interstitial transformation in gastric cancer SGC-7901/DDP cells
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- Author:
- No author available
- Journal Title:
- World Chinese Journal of Digestology
- Issue:
- 12
- DOI:
- 10.11569/wcjd.v27.i12.742
- Key Word:
- miR-214;胃癌;上皮间质转化;顺铂耐药
Abstract： 背景:胃癌(gastric cancer,GC)是常见的消化系统肿瘤之一,而肿瘤化疗耐药是制约GC癌治疗效果的瓶颈,探索逆转GC化疗耐药机制对于GC的治疗具有重大意义.目的:探讨敲低miR-214表达对GC细胞SGC-7901/DDP顺铂耐药性、迁移以及上皮间质转化(epithelial mesenchymal transformation,EMT)的影响,并讨论初步作用机制.方法:采用LipofectamineTM2000法转染miR-214 inhibitor至SGC-7901/DDP细胞后,CCK-8法评估细胞对顺铂耐药性的改变;划痕试验检测细胞迁移能力;免疫印迹法检测细胞中E-钙黏蛋白(E-cad)、波形蛋白(Vimentin)和N-钙黏蛋白(N-cad),以及NF-κB与Bcl-2蛋白表达水平.结果:转染miR-214 inhibitor后,SGC-7901/DDP细胞对顺铂耐药性明显降低(P＜0.05);且细胞迁移能力明显降低(P＜0.05),E-cad蛋白表达显著升高(P＜0.05),Vimentin 与N-cad蛋白表达均显著降低(均P＜0.05).另外,SGC-7901/DDP细胞中NF-κB与Bcl-2的表达明显降低(均P＜0.05).结论:下调miR-214表达具有降低GCSGC-7901/DDP细胞对顺铂耐药性,抑制细胞迁移与EMT的作用.而这种作用可能与下调NF-κB与Bcl-2的表达有关.
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