MiR-133 inhibits cell proliferation,migration, and invasion in gastric cancer cells by targeting JAK2
- Author:
- No author available
- Journal Title:
- World Chinese Journal of Digestology
- Issue:
- 35
- DOI:
- 10.11569/wcjd.v26.i35.2036
- Key Word:
- miR-133;胃癌细胞;JAK2;增殖;迁移;侵袭
Abstract： 目的 研究miR-133对胃癌(gastric cancer,GC)细胞增殖、迁移和侵袭的影响,并探讨其作用机制.方法 运用qRT-PCR法检测组织和细胞中miR-133、JAK2的mRNA表达;将miR-133组(转染miR-133 mimics)、miR-NC组(未转染细胞)、miR-133 inhibitors组(转染miR-133 inhibitors)、inhibitors-NC组(转染inhibitors)、JAK2 WT(载体psiCHECK2-JAK2-3'UTR WT和miR-133共转染)、JAK2 MUT(载体psiCHECK2-JAK2-3'UTR MUT和miR-133共转染)、miR-133+JAK2组(miR-133 mimics和JAK2共转染)、miR-133+Vector组(miR-133 mimics和pc-DNA 3.1共转染)均用脂质体法转染至AGS、MGC-803细胞;用Western blot检测细胞中JAK2的蛋白表达;MTT法检测细胞增殖;Transwell法检测细胞迁移和侵袭;双荧光素酶报告基因检测实验检测细胞荧光素酶活性.结果 与人癌旁组织、人正常胃黏膜细胞GES-1相比,GC组织、GC细胞AGS、MGC-803中miR-133均低表达,JAK2均高表达;且过表达miR-133、沉默JAK2均可抑制GC细胞增殖、迁移和侵袭;JAK2为miR-133的靶点,且回补JAK2可逆转miR-133对GC细胞增殖、迁移和侵袭的抑制作用.结论 miR-133可抑制GC细胞增殖、迁移和侵袭,其可能与靶向JAK2有关,将可为GC的临床诊断治疗提供新靶点.
- [1]李淑英,万福生.miRNA与肿瘤转移的研究进展[J].2018,(3).
- [2]王小磊,刘小方.miR-133在常见消化系统恶性肿瘤中的研究进展[J].2018,(16).
- [3]谭豆豆,马蓉,刘涛,等.p-JAK2蛋白在食管鳞状细胞癌中的表达及意义[J].2018,(3).
- [4]梁斌,王杉,叶颖江,等.JAK2激酶抑制剂AG490对胃癌细胞侵袭的影响[J].2004,(10).
- [5]Zhenguo Cheng,Funan Liu,Guanqiao Wang,etc.miR-133 is a key negative regulator of CDC42-PAK pathway in gastric cancer[J].2014,26(12).
- [6]Ling DING,Jianmin Si,Tianhua Zhou,etc.MiR-375 frequently downregulated in gastric cancer inhibits cell proliferation by targeting JAK2[J].2010,(7).
- [7]D S, Wang,H Q, Zhang,B, Zhang,etc.miR-133 inhibits pituitary tumor cell migration and invasion via down-regulating FOXC1 expression.[J].2016.
- [8]Stevan R, Hubbard.Mechanistic Insights into Regulation of JAK2 Tyrosine Kinase.[J].2017,8(Spec ).
- [9]Neudecker, Viola,Brodsky, Kelley S.,Kreth, Simone,etc.Emerging Roles for MicroRNAs in Perioperative Medicine[J].2016,124(2).
- [10]Wuping Li,Yun Zhong,Yuerong Shuang,etc.High concentration of miR-133 is a useful marker for the diagnosis of lymphoma-associated hemophagocytic syndrome.[J].2017,20(2).
- [11]Bo Xiao,Huazhen Liu,Zeyun Gu,etc.Expression of MicroRNA-133 Inhibits Epithelial-Mesenchymal Transition in Lung Cancer Cells by Directly Targeting FOXQ1[J].2016,(10).
- [12]Yanjun, Xu,Juan, Jin,Yiman, Liu,etc.Snail-regulated MiR-375 inhibits migration and invasion of gastric cancer cells by targeting JAK2.[J].2014,9.
- [13]Dang,S.,Hou,P.,Qu,Y..Gene methylation in gastric cancer[J].2013,424.
- [14]Liu, Yu,Han, Lili,Bai, Yahui,etc.Down-regulation of MicroRNA-133 predicts poor overall survival and regulates the growth and invasive abilities in glioma.[J].2018,46(1).
- [15]Zhang, Dongdong,Yang, Tianfeng,Kang, Yuan,etc.Novel compounds TAD-1822-7-F2 and F5 inhibited HeLa cells growth through the JAK/Stat signaling pathway[J].2018,103.
- [16]Li, Ting-Fu,Liu, Jian,Fu, Shi-Jie.The interaction of long non-coding RNA MIAT and miR-133 play a role in the proliferation and metastasis of pancreatic carcinoma[J].2018,104.
- [17]Yu,H.,Lu,Y.,Li,Z.,etc.microRNA-133: Expression, function and therapeutic potential in muscle diseases and cancer[J].2014,15(9).
- [18]Hua, Yu,Drew, Pardoll,Richard, Jove.STATs in cancer inflammation and immunity: a leading role for STAT3.[J].2009,9.
- [19]郭连英,高晓健,刘放,等.MiR-133与恶性肿瘤关系的研究进展[J].2015,(2).