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Comparison of tests for detecting Helicobacter pylori in gastric biopsy
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- Author:
- No author available
- Journal Title:
- World Chinese Journal of Digestology
- Issue:
- 25
- DOI:
- 10.11569/wcjd.v26.i25.1499
- Key Word:
- 胃幽门螺旋杆菌;免疫组织化学法;特殊染色;快速尿激酶法;荧光定量聚合酶链反应;胃癌
Abstract： 目的 分析比较四种胃活检幽门螺旋杆菌(Helicobacter pylori,Hpylori)检测方法,旨在找出一种敏感性,特异性高且操作便捷的检测方法.方法 选取490例胃黏膜活检组织标本,采用快速尿激酶法(rapid urease test,RUT)、荧光定量聚合酶链反应(fluorescence quantitative polymerase chain reaction,qPCR)、硼酸亚甲蓝法和免疫组织化学法(immunohistochemistry,IHC)进行检测,观察H.pylori的显示情况,对阳性率进行x2检验.结果 RUT、qPCR、硼酸亚甲蓝法和IHC在490例标本中的H.pylori阳性检出率分别为18.98％、63.88％、32.04％和38.16％,差异具有统计学意义(P＜0.005).结论 IHC检测H.pylori阳性组织,镜检为H.pylori呈黄褐色而周围胃黏膜组织细胞核呈蓝色,对比强烈,更为直观,有助于实验者获取具有潜在临床应用价值的形态学信息,且实验操作对仪器、实验人员无特殊要求.故建议将IHC检测胃活检H.pylor作为指导临床诊断H.pylori感染、实行H.pylori除菌治疗和疗效判断的依据之一.
- [1]ZHOU Liya,LIN Sanren,DING Shigang,etc.Relationship of Helicobacter pylori eradication with gastric cancer and gastric mucosal histological changes: a 10-year follow-up study[J].2014,(8).
- [2]H L Mobley,M D Island,R P Hausinger.Molecular biology of microbial ureases.[J].1995,59(3).
- [3]Ju Yup, Lee,Nayoung, Kim.Diagnosis of Helicobacter pylori by invasive test: histology.[J].2015,3(1).
- [4]中华医学会编著.临床技术操作规范[M].1900.
- [5]Takahiro, Uotani,David Y, Graham.Diagnosis of Helicobacter pylori using the rapid urease test.[J].2015,3(1).
- [6]She,Q.,Zhu,H.,Zhao,J.,etc.Diagnostic performance of urea breath test, rapid urea test, and histology for helicobacter pylori infection in patients with partial gastrectomy: A meta-analysis[J].2012,46(4).
- [7]Archimandritis A,Tzivras M,Sougioultzis S,etc.Rapid urease test is less sensitive than histology in diagnosing Helicobacter pylori infection in patients with non-variceal upper gastrointestinal bleeding.[J].2000,15(4).
- [8]Wen Zhong, Liu,Yong, Xie,Hong, Lu,etc.Fifth Chinese National Consensus Report on the management of Helicobacter pylori infection.[J].2018,(Spec ).
- [9]Ross, Dara S.,Landzberg, Brian R.,Jessurun, Jose,etc.Utility of ancillary stains for Helicobacter pylori in near-normal gastric biopsies[J].2015,46(3).
- [10]Wang XI,Zhang S,Abreo F,etc.The role of routine immunohistochemistry for Helicobacter pylori in gastric biopsy.[J].2010,14(4).
- [11]Pericak-Vance MA,Hedges DJ,Gilbert JR,etc.Sample degradation leads to false-positive copy number variation calls in multiplex real-time polymerase chain reaction assays[J].2009,386(2).