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Effect of "Biao-Ben points association" electro-acupuncture combined with dietary restriction on SIRT1 and UCP1 expression in brown adipose tissue of obese rats
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- Author:
- No author available
- Journal Title:
- World Chinese Journal of Digestology
- Issue:
- 22
- DOI:
- 10.11569/wcjd.v24.i22.3410
- Key Word:
- “标本配穴”电针;限食;肥胖;褐色脂肪;"Biao-Ben points association" electro-acupuncture therapy;Dietary restriction;Obesity;Brown adipose tissue
Abstract： 目的:研究“标本配穴”电针和饮食控制对肥胖大鼠褐色脂肪组织沉默信息调节因子1(silent information regulator1,SIRT1)和解偶联蛋白(uncoupling protein,UCP1)的影响,比较其单独及协同作用.方法:将40只高脂喂养的肥胖大鼠随机分为4组,每组10只.模型对照组(C)继续高脂喂养,电针组(E)给予“标本配穴”电针治疗,限食组(R)每只给予对照组摄食量的70％,电针+限食组(E+R)给予“标本配穴”电针结合限食干预治疗,共电针及限食8 wk.检测各组大鼠体质量和血清葡萄糖(fasting plasma glucose,FPG)、胰岛素(fasting insulin,FINS)、甘油三酯(triglyeride,TG)、总胆固醇(total cholesterol,TC)水平变化,对褐色脂肪组织进行SIRT1蛋白和UCP1mRNA的测定.结果:与C组比较,3个电针和饮食干预组体质量及FPG、FINS、TG和TC均明显下降(P＜0.01),且E+R组下降程度优于单独电针或限食组P＜0.05);与C组比较,3个干预组褐脂中SIRT1蛋白表达水平明显升高(P＜0.01);与C组比较,3个干预组褐脂中UCPl mRNA表达明显升高(P＜0.01),且E+R组效果明显优于单独电针或单独饮食干预组(P＜0.05).结论:“标本配穴”电针和限食可能是通过促进褐色脂肪组织中SIRT1和UCP1的表达,增加产热而发挥减肥作用.“标本配穴”电针法抵抗肥胖的功效与饮食控制相当,二者之间存在一定的协同作用.
- [1]王湛,曹宇.褐色脂肪组织代谢与肥胖[J].2011,(4).
- [2]刘志诚,孙凤岷,赵东红,等.针刺对肥胖大鼠褐色脂肪组织产热作用的影响[J].2003,(27).
- [3]徐芬,梁凤霞,陈瑞,等.标本配穴电针对胰岛素抵抗大鼠胰岛素敏感性及骨骼肌SIRT1蛋白表达的影响[J].2014,(4).
- [4]Weiguo, Dong,Wanqing, Quo,Feng, Wang,etc.Electroacupuncture Upregulates SIRT1-Dependent PGC-1α Expression in SAMP8 Mice.[J].2015,21.
- [5]Chengying, Gu,Yipeng, Zeng,Zhaosheng, Tang,etc.Astragalus polysaccharides affect insulin resistance by regulating the hepatic SIRT1-PGC-1α/PPARα-FGF21 signaling pathway in male Sprague Dawley rats undergoing catch-up growth.[J].2015,12(5).
- [6]冉红星,唐青青,王茵萍.耳甲部电针刺激对肥胖大鼠摄食和脂肪代谢的影响[J].2014,(3).
- [7]Ruth S M, Chan,Jean, Woo.Prevention of overweight and obesity: how effective is the current public health approach.[J].2010,7(3).
- [8]Philip V, Peplow,G David, Baxter.Electroacupuncture for control of blood glucose in diabetes: literature review.[J].2012,5(1).
- [9]Richard, Denis,Bakan, Inan,Caron, Alexandre,etc.In vivo measurement of energy substrate contribution to cold-induced brown adipose tissue thermogenesis[J].2015,29(5).
- [10]Xiao-Rong, Peng,Peter, Gennemark,Gavin, O'Mahony,etc.Unlock the Thermogenic Potential of Adipose Tissue: Pharmacological Modulation and Implications for Treatment of Diabetes and Obesity.[J].2015,6.
- [11]姚旋,张颖,单仕芳,等.褐色脂肪组织研究的最新进展和科学意义[J].2011,(3).
- [12]梁凤霞,陈瑞,王华.针灸防治胰岛素抵抗及其相关性疾病的临床研究思路探讨[J].2012,(7).
- [13]Koya D,Kume S,Liang F.SIRT1 and insulin resistance.[J].2009,5(7).
- [14]Lichtenbelt, Wouter D. van Marken,Schrauwen, Patrick.Implications of nonshivering thermogenesis for energy balance regulation in humans.[J].2011,70(2).
- [15]Guerra Sa, R.,Barbosa de Queiroz, K,Carvalho de Lima, D,etc.Endurance training blocks uncoupling protein 1 up-regulation in brown adipose tissue while increasing uncoupling protein 3 in the muscle tissue of rats fed with a high-sugar diet.[J].2012,32(9).
- [16]主编李忠仁.实验针灸学[M].2003.
- [17]Marc Uldry,Wenli Yang,Julie St-Pierre,etc.Complementary action of the PGC-1 coactivators in mitochondrial biogenesis and brown fat differentiation[J].2006,3(5).
- [18]王静芝,王华,周焕娇,等.电针对胰岛素抵抗大鼠下丘脑磷脂酰肌醇3激酶及磷脂酰肌醇3激酶催化亚基蛋白表达的影响[J].2014,(1).