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Optimal route of transplantation of bone marrow mesenchymal stem cells for therapy of acute pancreatitis

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Author:: No author available

Journal Title:: World Chinese Journal of Digestology

Issue:: 14

DOI:: 10.11569/wcjd.v24.i14.2152

Key Word:: 胰腺炎;骨髓间充质干细胞;移植途径;Pancreatitis;Bone marrow mesenchymal stem cells;Transplantation ways

Abstract：目的:探讨不同途径移植Pkh26标记的大鼠骨髓间充质干细胞(bone mesenchymal stem cell,BMSCs)治疗胰腺炎的最佳移植途径.方法:取3周龄健康SD♀小鼠的股骨及胫骨骨髓,进行分离纯化BMSCs.取体质量250-300 g♀SD大鼠160只,分模型组及治疗组,并在不同时间测大鼠的血清淀粉酶及血脂肪酶.将治疗组分为尾静脉组、胰腺局部注射组、肠系膜上静脉组,各实验组均移植红色荧光染料Pkh26标记的BMSCs.细胞移植术后12、24、48、72 h、1 wk测血清淀粉酶、血脂肪酶,3 wk观察Pkh26标记的BMSCs的定位及HE染色观察胰腺组织病理学变化.结果:细胞移植术后12、24、48、72h、1 wk 尾静脉注射组血清淀粉酶和血脂肪酶水平与模型组比较差异有统计学意义(P＜0.05),肠系膜上静脉组、胰腺局部注射组与相应的模型组比较显著下降(P＜0.05),胰腺局部注射组与肠系膜上静脉组比较显著下降(P＜0.05),但是仍高于对照组.尾静脉组各时间点胰腺内可见极少量Pkh26标记的BMSCs,肠系膜上静脉组2 wk时胰腺内可见散在亮红色荧光的细胞,数量少,3 wk时数量稍多,胰腺局部注射组2 wk及3 wk胰腺内均可见较多红色荧光的细胞,对照组各时间点均未见Pkh26标记细胞.结论:BMSCs经尾静脉、肠系膜上静脉、胰腺局部注射移植于胰腺炎大鼠模型体内,明显改善了受损胰腺的功能,对胰腺炎损伤具有修复作用,胰腺局部注射移植途径优于其他移植途径.

Reference

[1]金善丰,陈志耀,黄鹤光.绿色荧光蛋白转基因小鼠骨髓间充质干细胞干预重症急性胰腺炎大鼠后在多脏器的分布[J].2012,(10).
[2]胡菱,韩明子,薛洪璐,等.干细胞移植的安全性分析[J].2011,(1).
[3]Prockop DJ.Further proof of the plasticity of adult stem cells and their role in tissue repair.[J].2003,160(6).
[4]Karp JM,Leng Teo GS.Mesenchymal stem cell homing: the devil is in the details.[J].2009,4(3).
[5]Granger J,Remick D.Acute pancreatitis: models, markers, and mediators.[J].2005,24(Suppl.1).
[6]Meritxell Rovira,Fabien Delaspre,Mohammad Massumi,etc.Murine Embryonic Stem Cell-Derived Pancreatic Acinar Cells Recapitulate Features of Early Pancreatic Differentiation[J].2008,135(4).
[7]Otsuki M,Taguchi M,Yamaguchi T.Induction of PDX-1-positive cells in the main duct during regeneration after acute necrotizing pancreatitis in rats.[J].2002,197(5).
[8]Kudo M,Onda M,Suzuki T,etc.Defined localization of nestin-expressing cells in L-arginine-induced acute pancreatitis.[J].2006,32(4).
[9]Bradley EL 3rd,Dexter ND.Management of severe acute pancreatitis: a surgical odyssey.[J].2010,251(1).
[10]Raewyn M, Seaberg,Simon R, Smukler,Timothy J, Kieffer,etc.Clonal identification of multipotent precursors from adult mouse pancreas that generate neural and pancreatic lineages.[J].2004,22(9).
[11]Lin H.-T.,Otsu M.,Nakauchi H..Stem cell therapy: an exercise in patience and prudence[J].2012,368(1609).
[12]Guojian, Yin,Guoyong, Hu,Rong, Wan,etc.Role of bone marrow mesenchymal stem cells in L-arg-induced acute pancreatitis: effects and possible mechanisms.[J].2015,8(5).
[13]滕春燕,于庭,于艳辉,等.骨髓间充质干细胞对胰腺损伤模型大鼠血清生化指标的影响[J].2009,(3).
[14]Gong J,Tian F,Luo G,etc.Experimental evidence supporting the lack of primary stem cells in adult pancreatic tissue.[J].2010,10(5).
[15]Mitnala S,Pondugala PK,Guduru VR,etc.Reduced expression of PDX-1 is associated with decreased beta cell function in chronic pancreatitis.[J].2010,39(6).
[16]Gutowski KA,Hanson SE,Hematti P.Clinical applications of mesenchymal stem cells in soft tissue augmentation.[J].2010,30(6).
[17]Li,J.,Zhu,L.,Qu,X.,etc.Stepwise differentiation of human adipose-derived mesenchymal stem cells toward definitive endoderm and pancreatic progenitor cells by mimicking pancreatic development in Vivo[J].2013,22(10).
[18]Drapeau C,Jensen G.The use of in situ bone marrow stem cells for the treatment of various degenerative diseases.[J].2002,59(4).
[19]Schneider, Lutz,Jabrailova, Bahar,Strobel, Oliver,etc.Inflammatory profiling of early experimental necrotizing pancreatitis[J].2015,126.
[20]Song, Sun U.,Yi, TacGhee,Son, Mi Kwon,etc.Therapeutic effect of human clonal bone marrow-derived mesenchymal stem cells in severe acute pancreatitis[J].2015,38(5).
[21]D S, Krause,N D, Theise,M I, Collector,etc.Multi-organ, multi-lineage engraftment by a single bone marrow-derived stem cell.[J].2001,105(3).
[22]Zulewski H,Abraham EJ,Habener JF,etc.Multipotential nestin-positive stem cells isolated from adult pancreatic islets differentiate ex vivo into pancreatic endocrine, exocrine, and hepatic phenotypes.[J].2001,50(3).
[23]Nathalie, Swales,Geert A, Martens,Stefan, Bonné,etc.Plasticity of adult human pancreatic duct cells by neurogenin3-mediated reprogramming.[J].2012,7(5).
[24]RUPJYOTI TALUKDAR,SANTHI SWAROOP VEGE.Recent Developments in Acute Pancreatitis[J].2009,7(Suppl.1).
[25]Yu, Bai,Yan, Liu,Lin, Jia,etc.Severe acute pancreatitis in China: etiology and mortality in 1976 patients.[J].2007,35.
[26]陈珂玲,周总光,周斌.骨髓间充质干细胞旁分泌因子治疗重症急性胰腺炎的潜能[J].2015,(1).
[27]Enver Zerem.Treatment of severe acute pancreatitis and its complications[J].2014,(38).
[28]Li,L.,Li,F.-R.,Zhou,H.-X.,etc.Reversible immortalization of Nestin-positive precursor cells from pancreas and differentiation into insulin-secreting cells[J].2012,418(2).
[29]Gong,J.,Meng,H.-B.,Hua,J.,etc.The SDF-1/CXCR4 axis regulates migration of transplanted bone marrow mesenchymal stem cells towards the pancreas in rats with acute pancreatitis[J].2014,9(5).
[30]Schneider G,Saur D.Mesenchymal stem cells: therapeutic potential for acute pancreatitis.[J].2011,140(3).
[31]Swetha Kambhampati,Walter Park,Aida Habtezion.Pharmacologic therapy for acute pancreatitis[J].2014,(45).
[32]Xiao-Huang Tu,Jing-Xiang Song,Xiao-jun Xue,etc.Role of bone marrow-derived mesenchymal stem cells in a rat model of severe acute pancreatitis[J].2012,(18).