Influence of phosphatase of regenerating liver 3 on cell migration, invasion and expression of RhoC in SGC7901 cells
- Author:
- No author available
- Journal Title:
- World Chinese Journal of Digestology
- Issue:
- 12
- DOI:
- 10.11569/wcjd.v24.i12.1797
- Key Word:
- SGC7901;肝再生磷酸酶3;RhoC;SGC7901 cells;phosphatase of regenerating liver 3;RhoC
Abstract： 目的:通过观察肝再生磷酸酶3(phosphatase of regenerating liver 3,PRL-3)对胃癌SGC7901细胞迁移侵袭及RhoC表达的影响,探讨胃癌SGC7901细胞迁移侵袭的机制中PRL-3和RhoC是否位于同一信号通路.方法:体外培养人胃癌SGC7901细胞,经不同浓度PRL-3抗体(1∶600,1∶400,1∶200)作用后,采用细胞划痕实验观察不同时间段(0、12、24、48 h)SGC7901细胞的迁移距离;通过Transwell迁移侵袭实验检测不同浓度PRL-3抗体(1∶600,1∶400,1∶200)作用48h后SGC7901细胞迁移侵袭的穿膜细胞数,并采用Real-time PCR和ELISA检测SGC7901细胞中RhoC mRNA及蛋白表达量的变化,比较不同浓度PRL-3抗体处理后SGC7901细胞迁移侵袭的细胞数和RhoC mRNA及蛋白的表达量.结果:与对照组比较,随着PRL-3抗体浓度的升高和作用时间的延长,SGC7901细胞的迁移距离逐渐减小,在48 h,经不同浓度PRL-3抗体(1∶600,1∶400,1∶200)处理SGC7901细胞后,其迁移和侵袭的细胞数,RhoC mRNA相对表达量和蛋白表达水平与对照组相比均明显降低(迁移SGC7901细胞数:365.0±5.0,165.3±5.0,90.3±5.5 vs 512.3±4.9;侵袭SGC7901细胞数:321.3±6.1,179.0±6.1,75.7±4.0 vs 545.3±5.0;RhoC mRNA的相对表达量:0.910±0.022,0.742±0.018,0.539±0.015 vs l.000±0.000;RhoC蛋白表达量:1130.77 g/mL±15.32 g/mL,981.52 g/mL±14.44 g/mL,893.03 g/mL±11.10 g/mL vs 1212.42 g/mL±18.37g/mL)(均P＜0.01),不同PRL-3抗体浓度组两两比较均有统计学意义(均P＜0.01).结论:PRL-3在体外能显著促进SGC7901细胞的迁移侵袭,并能增强RhoC基因和蛋白的表达,提示在SGC7901细胞迁移侵袭的机制中PRL-3和RhoC可能位于同一信号通路.
- [1]张平,张志培,李香敏,等.PRL-3和RhoC在A549细胞中的表达及意义[J].2010,(12).
- [2]CAI Shi-rong,WANG Zhao,CHEN Chuang-qi,etc.Role of silencing phosphatase of regenerationg liver-3 expression by microRNA interference in the growth of gastric cancer[J].2008,(24).
- [3]张建龙,张萦斐,张育超,等.PRL-3调节PI3K信号通路在促进结肠癌细胞增殖侵袭中的作用[J].2013,(1).
- [4]Xiaofang, Xing,Shenyi, Lian,Ying, Hu,etc.Phosphatase of regenerating liver-3 (PRL-3) is associated with metastasis and poor prognosis in gastric carcinoma.[J].2013,11.
- [5]Yan, Wu,Yan, Tao,Yongchang, Chen,etc.RhoC regulates the proliferation of gastric cancer cells through interaction with IQGAP1.[J].2012,7(11).
- [6]Jian Ming,Yuo Jiang,Guocheng Jiang,etc.Phosphatase of Regenerating Liver-3 Induces Angiogenesis by Increasing Extracellular Signal-Regulated Kinase Phosphorylation in Endometrial Adenocarcinoma[J].2014,81(1).
- [7]Xian-Ying Fang,Ran Song,Wei Chen,etc.PRL-3 Promotes the Malignant Progression of Melanoma via Triggering Dephosphorylation and Cytoplasmic Localization of NHERF1.[J].2015,135(9).
- [8]宋倩,孟麟,曲立科,等.酪氨酸磷酸酶PRL-3增加人胃癌细胞BGC823的SP细胞比例并提高其对化疗药物的耐受性[J].2013,(3).
- [9]Zeng Q,Guo K,Wang H,etc.Monoclonal antibodies target intracellular PRL phosphatases to inhibit cancer metastases in mice.[J].2008,7(5).
- [10]Gu Y,Wang EH,Qiu X,etc.PRL-3 facilitates angiogenesis and metastasis by increasing ERK phosphorylation and up-regulating the levels and activities of Rho-A/C in lung cancer.[J].2009,41(2).
- [11]Min SH,Kim HM,Kim DM,etc.Downregulation of p53 by phosphatase of regenerating liver 3 is mediated by MDM2 and PIRH2.[J].2010,86(1).
- [12]James J, Fiordalisi,Patricia J, Keller,Adrienne D, Cox.PRL tyrosine phosphatases regulate rho family GTPases to promote invasion and motility.[J].2006,66(6).
- [13]Na Liu,Guoyun Zhang,Feng Bi,etc.RhoC is essential for the metastasis of gastric cancer[J].2007,85(10).
- [14]Hao, Liu,Abdul Qader Omer, Al-aidaroos,Haihe, Wang,etc.PRL-3 suppresses c-Fos and integrin α2 expression in ovarian cancer cells.[J].2013,13.
- [15]Ferlay, Jacques,Soerjomataram, Isabelle,Dikshit, Rajesh,etc.Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012[J].2015,136(5).
- [16]Haihe,Wang.PRL-3 down-regulates PTEN expression and signals through PI3K to promote epithelial-mesenchymal transition.[J].2007,67.
- [17]蒋蒙蒙,曹毅,李正荣,等.miR495、miR551a靶向干扰SGC7901细胞PRL-3基因的表达[J].2012,(34).
- [18]Liang F,Liang J,Wang WQ,etc.PRL3 promotes cell invasion and proliferation by down-regulation of Csk leading to Src activation.[J].2007,282(8).
- [19]许牧,曹毅,蒋蒙蒙,等.miR495、miR551a靶向干扰PRL-3表达抑制胃癌腹膜转移[J].2013,(18).
- [20]Ki-Woong Jeong,Dong-Il Kang,Eunjung Lee,etc.Structure and Backbone Dynamics of Vanadate-Bound PRL-3: Comparison of ~(15)N Nuclear Magnetic Resonance Relaxation Profiles of Free and Vanadate-Bound PRL-3[J].2014,53(29).
- [21]Lv,J.,Liu,C.,Huang,H.,etc.Suppression of breast tumor growth by DNA vaccination against phosphatase of regenerating liver 3[J].2013,20(8).
- [22]Wenhua, Zhou,Chi, Zhang,Hao, Jiang,etc.MiR-493 suppresses the proliferation and invasion of gastric cancer cells by targeting RhoC.[J].2015,18.
- [23]S, Saha,A, Bardelli,P, Buckhaults,etc.A phosphatase associated with metastasis of colorectal cancer.[J].2001,294(5545).