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Treatment and detection of mycoplasma contamination during cell culture
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- Author:
- No author available
- Journal Title:
- World Chinese Journal of Digestology
- Issue:
- 10
- DOI:
- 10.11569/wcjd.v24.i10.1557
- Key Word:
- 支原体;细胞培养;防治;检测;Mycoplasma;Cell culture;Treatment;Detection
Abstract： 目的:为高效便捷地去除细胞培养过程中支原体污染,采用多种抗生素和检测方法对污染细胞进行处理和检测.方法:分别采用Plasmocin、BM-Cyclin、MRA3种药物对污染细胞进行处理,以CLARK的一步法试剂、Biotool的快速检测试剂和自行研制的聚合酶链式反应(polymerase chain reaction,PCR)等3种方法进行检测.结果:一步法试剂检测显示Plasmocin处理14 d后支原体消除,快速检测试剂检测显示BM-Cyclin处理21 d彻底消灭支原体,而MRA经PCR检测发现彻底消除支原体需要14 d;另外,3种药物交替使用清除支原体的效果更加显著.结论:通过使用3种抗生素和3种支原体检测方法,使细胞培养过程中的支原体污染得到有效控制,为更好地开展细胞研究奠定基础.
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