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Correlation of serum leptin with oncogene expression in colorectal cancer
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 14
- DOI:
- 10.3969/j.issn.1009-3079.2010.14.020
- Key Word:
- 结直肠癌;瘦素;癌基因
Abstract： 目的:研究血清瘦素与结直肠癌突变癌基因的相关性,探讨其参与结直肠癌发生发展的机制.方法:研究对象肿瘤组选择30例结直肠癌患者,选择24名健康成年人作为对照组.用双抗体夹心ABC-ELISA法测定血清的瘦素水平,采用RT-PCR方法测定肿瘤标本的癌基因K-ras以及抑癌基因P53、APC和DCC的表达,对各癌基因表达的差异与血清的瘦素水平做出相关性分析.结果:肿瘤APC抑癌基因表达阳性的患者血清瘦素水平要高于表达阴性者(3.78μg/L±1.85μg/L vs 2.41 μg/L±2.53 μg/L,P<0.05),且随着血清瘦素水平的升高APC基因的表达强度也逐渐增加(P<0.05),其余3种癌基因的表达与血清瘦素水平没有明显相关性(P>0.05).结论:瘦素参与结直肠癌发生发展的途径与抑癌基因APC的表达有关,与癌基因K-ras及抑癌基因P53、DCC的表达无关.
- [1]Lu S,Archer MC,Ealey KN.Development of aberrant crypt foci in the colons of ob/ob and db/db mice: evidence that leptin is not a promoter.[J].2008,47(9).
- [2]O, Tetsu,F, McCormick.Beta-catenin regulates expression of cyclin D1 in colon carcinoma cells.[J].1999,398(6726).
- [3]Kaaks R,Stenman UH,Bergh A,etc.Leptin is associated with increased prostate cancer risk: a nested case-referent study.[J].2001,86(3).
- [4]Roger K Wolff,Martha L Slattery.Leptin and colorectal cancer: an undefined link[J].2007,4(3).
- [5]Y, Zhang,R, Proenca,M, Maffei,etc.Positional cloning of the mouse obese gene and its human homologue.[J].1994,372(6505).
- [6]Liu Z,Kato N,Uesaka T,etc.High fat diet enhances colonic cell proliferation and carcinogenesis in rats by elevating serum leptin.[J].2001,19(5).
- [7]Desai KM,Randeva HS,Mistry T,etc.Leptin and adiponectin interact in the regulation of prostate cancer cell growth via modulation of p53 and bcl-2 expression.[J].2008,101(10).
- [8]Cho JY,Kwon KW,Lee MS,etc.Variation in expression of gastric leptin according to differentiation and growth pattern in gastric adenocarcinoma.[J].2006,33(2).
- [9]T C, He,A B, Sparks,C, Rago,etc.Identification of c-MYC as a target of the APC pathway.[J].1998,281.
- [10]De-Johnston J,Funanage VL,Smith-Kirwin SM,etc.Leptin expression in human mammary epithelial cells and breast milk.[J].1998,83(5).
- [11]Hord NG,Fenton JI,Perkins SN,etc.Microarray analysis reveals that leptin induces autocrine/paracrine cascades to promote survival and proliferation of colon epithelial cells in an Apc genotype-dependent fashion.[J].2008,47(1).
- [12]Garofalo C,Surmacz E.Leptin and cancer.[J].2006,207(1).