You Position: Home > Paper

Serum from rats medicated with Dachengqitang significantly enhances the expression of inositol 1, 4, 5-triphosphate receptor mRNA in interstitial cells of Cajal in rat jejunum

( views:0, downloads:0 )
Author:
No author available
Journal Title:
WORLD CHINESE JOURNAL OF DIGESTOLOGY
Issue:
27
DOI:
10.3969/j.issn.1009-3079.2009.27.012
Key Word:
大承气汤;Cajal间质细胞;三磷酸肌醇受体

Abstract´╝Ü 目的:探讨大承气汤含药动物血清对大鼠胃肠Cajal间质细胞(ICC)三磷酸肌醇受体(IP3R)表达的影响.方法:制备大承气汤含药动物血清,作用于Wistar大鼠空肠ICC细胞,然后RT-PCR检测空白培养液组、含药血清高浓度组(100%含药血清DMEM-F12培养液)及低浓度组(20%含药血清DMEM-F12培养液)IP3R受体表达变化.结果:大鼠空肠ICC细胞3型IP3R均有表达,含药动物血清组较正常血清组明显增加(均P<0.05),高浓度组较低浓度组有显著差异(IP3R1/β-actin:1.0124±0.129 vs 0.625±0.075;IP3R2/β-actin:0.813±0.098 Vs 0.476±0.031;IP3R3/β-actin:0.924±0.113 vs 0.583±0.046,均P<0.05).结论:大承气汤动物含药血清可显著增强大鼠小肠ICC IP3R表达,并存在量效依赖关系.

WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn