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Serum from rats medicated with Dachengqitang significantly enhances the expression of inositol 1, 4, 5-triphosphate receptor mRNA in interstitial cells of Cajal in rat jejunum
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 27
- DOI:
- 10.3969/j.issn.1009-3079.2009.27.012
- Key Word:
- 大承气汤;Cajal间质细胞;三磷酸肌醇受体
Abstract： 目的:探讨大承气汤含药动物血清对大鼠胃肠Cajal间质细胞(ICC)三磷酸肌醇受体(IP3R)表达的影响.方法:制备大承气汤含药动物血清,作用于Wistar大鼠空肠ICC细胞,然后RT-PCR检测空白培养液组、含药血清高浓度组(100%含药血清DMEM-F12培养液)及低浓度组(20%含药血清DMEM-F12培养液)IP3R受体表达变化.结果:大鼠空肠ICC细胞3型IP3R均有表达,含药动物血清组较正常血清组明显增加(均P<0.05),高浓度组较低浓度组有显著差异(IP3R1/β-actin:1.0124±0.129 vs 0.625±0.075;IP3R2/β-actin:0.813±0.098 Vs 0.476±0.031;IP3R3/β-actin:0.924±0.113 vs 0.583±0.046,均P<0.05).结论:大承气汤动物含药血清可显著增强大鼠小肠ICC IP3R表达,并存在量效依赖关系.
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