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Phenylpropanoid glycoside reverse multidrug resistance of colon carcinoma LoVo/Adr cells through induction of apoptosis

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Author:: No author available

Journal Title:: WORLD CHINESE JOURNAL OF DIGESTOLOGY

Issue:: 23

DOI:: 10.3969/j.issn.1009-3079.2009.23.005

Key Word:: 苯丙素甙;多药耐药;逆转;凋亡

Abstract：目的: 探讨苯丙素甙化合物逆转大肠癌耐药与凋亡的关系.方法: 实验分3组: LoVo/Adr细胞组(空白对照组);LoVo/Adr细胞+PPG 40 mg/L组(实验组);LoVo/Adr细胞+VP 5 mg/L(阳性对照组). 采用MTT法检测苯丙素甙对LoVo/Adr细胞的耐药性;流式细胞仪分析苯丙素甙对大肠癌耐药LoVo细胞的凋亡的作用;通过检测pNA量观察苯丙素甙对LoVo/Adr细胞Caspase-3的活性.结果: 苯丙素甙可降低阿霉素对LoVo/Adr细胞的IC50值, 具有逆转作用, 其逆转倍数为9.93;他还可引起LoVo/Adr细胞凋亡, 与对照组相比, 具有显著性差异(P <0.01). 经苯丙素甙作用后, LoVo/Adr细胞pNA释放量明显增加(31.75±4.34 pmol/min vs 18.45±2.39 pmol/min,P <0.01), 而同时加入Caspase-3抑制剂Z-VADFMK,pNA释放量明显低于实验组(17.69±2.68 pmol/min vs 31.75±4.34 pmol/min,P <0.01).结论: 苯丙素甙对耐药LoVo/Adr细胞具有逆转耐药作用, 通过Caspase-3途径诱导凋亡可能为其逆转耐药的机制之一.

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