Feasibility of a polysaccharide isolated from Bletilla striata used as a gene vector administered through an interventional pathway
- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 18
- DOI:
- 10.3969/j.issn.1009-3079.2009.18.006
- Key Word:
- 介入;白芨多糖;基因载体;胺化还原法;转染;Interventional;Bletilla striata polysaccharide;Gene vector;Reductive amination;Transfection
Abstract： AIM: To research into the feasibility of a polysaccharide isolated from Bletilla striata as a gene vector administered through an interventional pathway. METHODS: Bletilla striata polysaccharide (BSP) was isolated from a Chinese medicinal herb Bletilla striata, from which we prepared the cationic BSP (BSP+) by reductive amination. We further tested the ability of the BSP+ to incorporate and protect plasmid DNA, and the ability of the BSP+/pDNA complex to transfect into HepG2 cells. Then, we investigated the transfected effect of the BSP+/pDNA complex in vivo using green fluorescence protein (GFP) gene as a reporter gene administered through the hepatic artery. RESULTS: The synthesized cationic Bletilla striata polysaccharide (BSP+) incorporated and protected plasmid DNA to avoid enzymolysis by Dnase. The transfection ratio was lower in the liposome group than in BSP+ group (28.87% ± 3.27% vs 36.64% ± 6.87%, P < 0.05). The BSP+/pDNA complex was transfected into HepG2 cells in vitro and host cell in vivo. CONCLUSION: The BSP+/pDNA complex could target transfect into liver cell in vivo when administered through the hepatic artery using the interventional method, which could produce a marked effect as a new-type polycation gene vector in gene therapy.
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