Influence of Bifidobacterium on gut barrier function and Th1/Th2 cytokine in food allergic mice
- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 11
- DOI:
- 10.3969/j.issn.1009-3079.2009.11.006
- Key Word:
- 食物过敏;肠道菌群;细菌移位;肠通透性;CD4+CD25+调节性T细胞;Th1/Th2
Abstract： 目的:探讨双歧杆菌在改善食物过敏动物肠道屏障功能、调整肠道菌群结构以及对免疫功能调节方面的作用及其机制.方法:无受试蛋白喂养BALB/c小鼠40只,随机分为4组: 分别于0、3、9 d腹腔注射生理盐水,金黄色葡萄球菌肠毒素B(SEB),卵清蛋白(OVA),SEB+OVA; 并于第7、14天给予OVA灌胃.在SEB+OVA致敏组的基础上设立自然恢复组、双歧杆菌作用组、思密达作用组、双歧杆菌+思密达共同作用组,第15天开始分别经灌胃给予不同的药物,连续7 d,每日1次.培养法分析粪便菌群,检测血清二胺氧化酶(DAO)含量,ELISA法测定血清IgE、IL-4、INF-γ含量.对肠系膜淋巴结(MLN)及肝、肾、肺组织进行培养以探讨有无细菌移位(BT)发生.同时,采用流式细胞术分析其脾细胞悬液中CD4+CD25+调节性T细胞的数量变化.结果:与SEB+OVA实验组相比,双歧杆菌作用组小鼠血清IgE、DAO含量(A 值)、血清IL-4(51.314±3.785 ng/L vs 69.980±9.103ng/L,P<0.05)含量显著降低; 血清INF-γ水平显著升高(194.281±12.144 ng/L vs 133.875±33.822 n g/L,P<0.05); 脾细胞悬液中CD4+CD25+T细胞数量显著升高(5.778%±0.773% vs 4.216%±0.439%,P<0.05); 肠道固有菌群中益生菌乳酸杆菌的含量(6.670±0.443 vs 5.654±0.289,P<0.05)、双歧杆菌的含量(8.611±0.295 vs 7.491±0.339,P<0.05)显著升高,条件致病菌大肠杆菌的含量(5.364±0.537 vs 6.718±0.267,P<0.05)、类杆菌的含量(7.427±0.544 vs 8.606±0.317,P<0.05)显著降低; MLN及外周器官细菌移位率显著降低(12.5% vs 37.5%,P<0.05).结论:双歧杆菌可以有效调节机体免疫功能、调整肠道菌群失调及保护肠道黏膜屏障功能.
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