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Early growth response factor 1 expression in rat lungs and alveolar macrophages during acute pancreatitis

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Author:: No author available

Journal Title:: WORLD CHINESE JOURNAL OF DIGESTOLOGY

Issue:: 35

DOI:: 10.3969/j.issn.1009-3079.2007.35.005

Key Word:: 急性胰腺炎;早期生长反应基因-1;肺损伤;弹性蛋白酶;肺泡巨噬细胞;细胞外信号调节激酶;逆转录聚合酶链反应

Abstract：目的:研究观察早期生长反应基因-1(EGR-1)在急性胰腺炎(AP)大鼠肺组织与原代培养肺泡巨噬细胞(AM)中表达的作用.方法:将♂Wistar大鼠40只,随机均分为4组,分别于胆总管内逆行注入生理盐水或不同浓度牛磺胆酸钠溶液.3 h后处死动物,取血分离血清,并剪取胰腺及肺组织.检测血清TNF-α和IL-1β水平,胰腺组织病理评分,肺组织湿质量/干质量比,并对肺组织进行EGR-1免疫组化染色.原代培养AM分成4组,分别采用不同浓度胰弹性蛋白酶进行刺激,免疫细胞化学染色法检测EGR-1在AM的表达,并检测培养液中TNF-α和IL-1 β浓度.RT-PCR分别检测AM,EGR-1,TNF-α,IL-1 β mRNA表达.结果:肺组织EGR-1表达随AP病情加重而呈增强趋势,并与血清TNF-α和IL-1β水平、胰腺组织病理评分、肺组织湿质量/干质量比均呈显著正相关(r=0.63,0.58,0.59,0.61,P＜0.01)AM中EGR-1蛋白.mRNA表达程度与TNF-α和IL-1β蛋白(r=0.64、0.51,P＜0.01)以及mRNA水平均呈显著正相关(r=0.62,0.59,P＜0.01).结论:EGR-1可能在AP并发肺损伤中起重要作用,其机制可能与其介导炎性细胞因子生成有关.

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