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Expression of the caspase 9 gene and its polymorphism distribution in gastric cancer
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 30
- DOI:
- 10.3969/j.issn.1009-3079.2007.30.008
- Key Word:
- 胃癌;Caspase 9;单核苷酸多态;限制性片段长度多态性技术
Abstract： 目的:探讨胃癌中caspase 9基因的表达及其单核苷酸多态位点的分布.方法:应用Western-blot方法检测70例胃癌患者及对应癌旁正常组织中caspase 9的表达情况.应用限制性片段长度多态性技术结合测序,分析70例胃患者及100例正常人caspase 9基因rs1052576位点基因型.应用列联表法统计分析患者组和对照组SNP位点基因型及等位基因频率.结果:51.4%(36/70)的胃癌组织caspase 9基因表达明显下调,与癌旁正常组织相比差异有统计学意义(P＜0.05).位于caspase 9基因第5外显子的SNP位点rs1052576的基因型频率和等位基因频率在两组人群中的分布差异有统计学意义.患者组G等位基因频率明显高于对照组(22.9% vs 13.0%,P＜0.05),AG基因型频率在有淋巴结转移的患者中明显高于无淋巴结转移的患者(P＜0.05).结论:caspase 9基因在胃癌中低表达,rs1052576多态位点G等位基因和胃癌的发生相关,AG基因型和淋巴结转移有关.
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