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Role of nitric oxide in the protective effect of rabeprazole on gastric mucosal lesions in rats
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 28
- DOI:
- 10.3969/j.issn.1009-3079.2006.28.012
- Key Word:
- 一氧化氮;雷贝拉唑;胃黏膜损伤;细胞保护
Abstract： 目的:探讨一氧化氮(NO)在雷贝拉唑对大鼠胃黏膜损伤保护中的作用.方法:在乙醇诱导大鼠胃黏膜损伤前,预先给予雷贝拉唑(20 mg/kg)灌胃,1-硝基-精氨酸甲酯(1-NAME 4 mg/kg)、1-精氨酸(250 mg/kg)及d-精氨酸(250 mg/kg)iv.采用激光多普勒血流计(LDF)测定胃黏膜血流量(GMBF),采用镉粒还原和比色法测定胃黏膜和血浆NO-2/NO-3含量,并观察胃黏膜损伤指数(UI)、溃疡坏死组织和中性粒细胞浸润严重程度的变化.结果:与模型损伤组比,雷贝拉唑组大鼠UI明显降低(5.5±0.5 vs 25.2±2.3,P＜0.01),溃疡坏死组织和中性粒细胞浸润程度明显减轻(坏死物质++→+++/≤+:1/9 vs 8/2,P＜0.01;中性粒细胞++→+++≤+:3/7 vs 9/1,P＜0.01).预先用1-NAME处理后,雷贝拉唑保护胃黏膜损伤作用明显减弱;1-NAME抑制作用可补1-精氨酸拮抗,而不被d-精氨酸拮抗.向胃内灌注雷贝拉唑,可增加GMBF、胃黏膜和血浆NO-2/NO-3,1-NAME可逆转这种作用,但对雷贝拉唑抑制酸分泌作用无明显影响.结论:雷贝拉唑对大鼠胃黏膜损伤保护作用与NO有关,而与雷贝拉唑抑制酸分泌作用无关.
- [1]乐桥良,文晓冬.大鼠应激性溃疡中一氧化氮的作用[J].2000,(7).
- [2]乐桥良,张志坚,林克荣,等.潘托拉唑对胃黏膜损伤保护作用及其机制[J].2005,(20).
- [3]Joshua Ka-Shun Ko,Chi-Hin Cho,Shiu-Kum Lam.Adaptive cytoprotection through modulation of nitric oxide in ethanol-evoked gastritis[J].2004,(17).
- [4]R J F, Laheij,L G M, Van Rossum,J B M J, Jansen,etc.Proton-pump inhibitor therapy for acetylsalicylic acid associated upper gastrointestinal symptoms: a randomized placebo-controlled trial.[J].2003,18(1).
- [5]Motilva V,Herrerias JM,Ortiz P,etc.Role of prostaglandins and nitric oxide in gastric damage induced by metamizol in rats.[J].2002,51(8).
- [6]Shoda R,Muraoka A,Yamato S,etc.Nitric oxide donating compounds inhibit HCl-induced gastric mucosal lesions mainly via prostaglandin.[J].2001,85(2).
- [7]Spirt MJ.Stress-related mucosal disease: risk factors and prophylactic therapy.[J].2004,26(2).
- [8]Suzuki H,Nagata H,Sato M,etc.Rabeprazole treatment attenuated Helicobacter pylori-associated gastric mucosal lesion formation in Mongolian gerbils.[J].2003,18(7).
- [9]Kahrilas PJ,Pandolfino JE,Zhang Q,etc.Four-day Bravo pH capsule monitoring with and without proton pump inhibitor therapy.[J].2005,3(11).
- [10]Maciej, Swiatkowski,Jacek, Budzyński,Maria, K?opocka,etc.The effect of eight weeks of rabeprazole therapy on nitric oxide plasma level and esophageal pH and motility and motility nitric oxide plasma level in patients with erosive esophagitis.[J].2004,10.
- [11]Banerjee SN,Pal TK,Jayakumar M,etc.Bioequivalence study of rabeprazole sodium on healthy human volunteers.[J].2004,102(1).
- [12]Mansi C,Santi ML,Bilardi C,etc.A study of 4- and 7-day triple therapy with rabeprazole, high-dose levofloxacin and tinidazole rescue treatment for Helicobacter pylori eradication.[J].2006,23(2).
- [13]Brown NJ,Jacob S,Reed MW,etc.L-Arginine protects and exacerbates ethanol-induced rat gastric mucosal injury.[J].2000,15(8).
- [14]Itagaki F,Homma M,Yuzawa K,etc.Effect of lansoprazole and rabeprazole on tacrolimus pharmacokinetics in healthy volunteers with CYP2C19 mutations.[J].2004,56(8).
- [15]Ohta Y,Nishida K.Protective effect of L-arginine against stress-induced gastric mucosal lesions in rats and its relation to nitric oxide-mediated inhibition of neutrophil infiltration.[J].2001,43(6).
- [16]Santarelli L,Gabrielli M,Candelli M,etc.Post-cholecystectomy alkaline reactive gastritis: a randomized trial comparing sucralfate versus rabeprazole or no treatment.[J].2003,15(9).
- [17]Samini M,Jabarizadeh N,Shafaroodi H,etc.Evidences for involvement of nitric oxide in the gastroprotective effect of bromocriptine and cyclosporin A on water immersion stress-induced gastric lesions.[J].2002,46(6).
- [18]Shimatani T,Daimaru Y,Yokoya H,etc.Evaluation of gastric acid secretion in two patients (each aged over 90 years) with Helicobacter pylori-negative nonsteroidal anti-inflammatory drug-caused duodenal ulcers.[J].2003,38(2).
- [19]Tominaga K,Watanabe T,Arakawa T,etc.Cytoprotective effect of rabeprazole against ethanol-induced gastric mucosal damage: possible involvement of nitric oxide.[J].2000,26(2).