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In vitro effects of lamivudine on function of dendritic cells derived from patients with chronic hepatitis B infection
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 17
- DOI:
- 10.3969/j.issn.1009-3079.2006.17.008
- Key Word:
- 慢性乙型肝炎;树突状细胞;抗原提呈;拉米夫定
Abstract： 目的:体外研究不同浓度拉米夫定(lamivudine,LAM)对慢性乙型肝炎(chronic hepatitis B,CHB)患者树突状细胞(dendritic cell,DC)功能影响.方法:分离慢乙肝患者外周血单核细胞,在含GM-CSF+IL-4及不同浓度LAM(0,0.125,0.25,0.5,1,2 mmol/L)培养条件下制备DC.观察DC形态学变化并用MTT法测定DC刺激同种异体淋巴细胞增殖能力,流式细胞仪(FCM)测定其细胞表型分子CD1a,CD83,CD80及HLA-DR的表达,ELISA法检测培养上清中IL-12和IL-6含量.结果:在LAM 0.5 mmol/L组,DC表型分子CD83,CD1a,HLA-DR的表达最高,CD80与LAM未处理组相比无明显差异.与LAM未处理组相比,LAM 0.5 mmol/L处理组DC膜表面分子CD1a,CD83,HLA-DR表达增高(CD1a:54.0±9.2 vs 33.6±10.1,P＜0.05;CD83:20.3±6.1 vs 11.8±6.2,P＜0.05;HLA-DR:74.5±7.1 vs 52.9±7.7,P＜0.05);其上清液中IL-12分泌水平增高(810.0±91.5 ng/L vs 268.0±34.3 ng/L,P＜0.05),IL-6则显著降低(28.1±2.6 ng/L vs 55.3±7.4 ng/L,P＜0.05);刺激同种异体淋巴细胞增殖能力(SI)增强(1.9±0.6 vs 1.2±0.5,P＜0.05).结论:LAM体外可增强慢乙肝患者树突状细胞活性.
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