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Expression of tumor necrosis factor-α and c-fos mRNA in immature rats' model of gut ischemia/reperfusion injury
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 16
- DOI:
- 10.3969/j.issn.1009-3079.2006.16.017
- Key Word:
- 肠;缺血/再灌注;肿瘤坏死因子;c-fos基因
Abstract： 目的:观察幼鼠肠缺血/再灌注损伤(I/R)不同时点肠组织内肿瘤坏死因子α(TNF-α)、c-fosmRNA表达模式,探讨肠I/R的机制.方法:分离幼鼠肠系膜上动脉,制作动物模型,应用RT-PCR半定量法分析肠组织TNF-α、c-fosmRNA表达.结果:与假手术组相比,肠组织内TNF-αmRNA缺血30 min显著升高(1.55±0.33 vs1.07±0.08,P＜0.05),再灌注30 min达峰值(3.05±0.11),再灌注后60,90 min逐渐下降;c-fosmRNA表达于缺血30 min后升高(0.95±0.13vs 0.12±0.02,P＜0.05),再灌注30 min达峰值(1.53±0.11),再灌注后60 min迅速下降,再灌注后90 min达基线水平.结论:幼鼠肠I/R损伤介导肠组织内TNF-α和c-fos mRNA的表达模式.c-fos mRNA表达伴随TNF-αmRNA表达,提示二者间可能存在关系.
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