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Effects of HBeAg and HBsAg on cytokine excretion and angiotensin-converting enzyme activity in HepG2 cell line
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 9
- DOI:
- 10.3969/j.issn.1009-3079.2006.09.013
- Key Word:
- HBV;肝纤维化;血管紧张素转化酶;HepG2
Abstract： 目的:观察HBeAg和HBsAg对人肝癌细胞系HepG2细胞中血管紧张素转化酶(ACE)分泌的影响.方法:利用本所既往构建的表达乙肝病毒(HBV)表面抗原和E抗原的真核表达载体pcDNA3.1(-)-HBsAg、pcDNA3.1(-)-HBeAg及空载体pcDNA3.1(-)转染HepG2细胞,观察转染24,48 h后细胞上清中ACE活性和炎性细胞因子IL-6、IL-8的变化.培养细胞上清液内ACE活性采用底物裂解法检测;IL-6、IL-8浓度采用流式细胞仪检测.结果:转染48 h的HepG2细胞裂解液中检测到了HBsAg和HBeAg的表达,但二者细胞上清中的ACE与转染空载体对照pcDNA3.1(-)细胞上清中的ACE无显著差异;同样,炎性因子IL-6和IL-8也没有显著差异.结论:HBV HBsAg和HBeAg对ACE活性、IL-6和IL-8不具有显著的上调或下调作用;HBsAg和HBeAg在肝纤维化发生过程中的作用机制尚待进一步阐明.
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