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Clinical significance and expression of cytokine-induced neutrophil chemoattractant in severe acute pancreatitis
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 22
- DOI:
- 10.3969/j.issn.1009-3079.2005.22.017
- Key Word:
- 重症急性胰腺炎;中性粒细胞趋化因子;发病
Abstract： 目的:探讨中性粒细胞趋化因子(CINC)在重症急性胰腺炎(SAP)发病机制中的作用.方法:采用向胆胰管逆行注射50 g/L牛磺胆酸钠建立大鼠SAP模型,48只♂ SD大鼠随机分为手术对照组和SAP组,分别检测各组不同时间点血清淀粉酶和胰腺组织病理学改变,用免疫组织化学法和半定量逆转录聚合酶链反应(RT-PCR)法检测胰腺组织中CINC蛋白和CINC mRNA表达的变化情况.结果:牛磺胆酸钠组与手术对照组相比,大鼠的血清淀粉酶明显增高、胰腺组织学改变明显;术后1 h胰腺腺泡细胞CINC蛋白和胰腺组织中CINC mRNA的表达高于手术对照组,随着时间的延长表达逐渐增强,牛磺胆酸钠组术后1 h胰腺腺泡细胞CINC mRNA表达较手术对照组明显增高分别为(0.37±0.10 vs 0.29±0.10,P＜0.05),并且随着时间的延长差别更加明显:免疫组化显示CINC蛋白表达也高于手术对照组.结论:CINC可能在SAP发病过程中起了重要的作用.
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