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Drug-resistant mutation and genotype of hepatitis B virus Heilongjiang Province
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 14
- DOI:
- 10.3969/j.issn.1009-3079.2005.14.012
- Key Word:
- 乙型肝炎;基因型;拉米夫定;YMDD基因变异;耐药
Abstract： 目的:探讨黑龙江地区乙型肝炎病毒发生YMDD基因变异与HBV基因型的关系.方法:首先HBVA-F6个主要基因型特异性多引物对巢式PCR法对106例接受拉米夫定抗病毒治疗患者的血清HBV DNA进行基因分型和分析.再采用实时荧光PCR法对标本进行YMDD变异检测.结果:在106例标本中有8例为B基因型,其阳性率为7.6%;有94例为C基因型,其阳性率为88.7%;4例为D基因型,其阳性率为3.8%.从106例血清标本中检测出63例YMDD发生变异占59.4%,其余43例为野生型YMDD.在8例B基因型的标本中,有5例发生YMDD变异,变异率为62.5%;在94例C基因型的标本中,有55例发生YMDD变异,变异率为58.5%.HBV B,C两基因型间的YMDD变异率无显著性差异(P>0.05).在5例B基因型YMDD变异的标本中,有1例发生YIDD变异(20%),其余4例发生YVDD(YMDD+YVDD)变异占80%;在55例C基因型YMDD变异的标本中,有26例发生YIDD(YMDD+YIDD)变异占47.3%,17例发生YVDD(YMDD+YVDD)变异占30.9%,12例发生YIDD+YVDD变异占21.8%.HBVB,C两基因型间的YMDD不同变异类型发生率无显著性差异(P>0.05).结论:在黑龙江地区拉米夫定抗病毒治疗2-4 a,HBVYMDD变异率为59.4%;HBV B,C基因型标本间发生YMDD变异率及基因变异类型均无显著性差异.
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