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Effect of amino-D-glucose derivative on proliferation of human gastric carcinoma cell line SGC-7901 in vitro
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 6
- DOI:
- 10.3969/j.issn.1009-3079.2005.06.005
- Key Word:
- D-氨基葡萄糖;胃癌;SGC-7901细胞
Abstract： 目的:观察D-氨基葡萄糖衍生物体外对人胃癌SGC-7901细胞增生的影响,探讨其可能作用机制.方法:采用MTT法,筛选药物作用最佳浓度.用流式细胞仪分析诱导凋亡前后的细胞DNA含量,透射电镜观察凋亡细胞的超微结构.免疫组化测定CD44表达.结果:D-氨基葡萄糖衍生物能明显抑制胃癌细胞的增长(P＜0.01),MTT法显示抑制程度具有时间和剂量效应关系,统计组间比较差异有显著性(P＜0.01);流式细胞仪分析可见亚二倍体(Sub-G1)凋亡峰;电镜观察到凋亡细胞的典型变化,免疫组化及光密度检测示CD44表达下降(216.5±7.0 vs 190.0±14.2,P=0.000).结论:D-氨基葡萄糖衍生物通过诱导肿瘤细胞凋亡而抑制人胃癌SGC-7901细胞增生,同时还有下调CD44的作用.
- [1]顾正勤,孙颖浩,许传亮,等.黄芩苷诱导前列腺癌细胞株DU145凋亡的体外研究[J].2005,(1).
- [2]邢承忠,陈峻青.细胞内游离Ca2+和cAMP浓度在胃癌细胞凋亡过程中的变化特点[J].1999,(5).
- [3]谢秋玲,戴云,孙奋勇,等.盐酸氨基葡萄糖胺诱导黑素瘤细胞分化的形态学观察[J].2002,(3).
- [4]王哲,乔岩,黄高昇,等.N-乙酰氨基葡萄糖诱导白血病细胞K562向巨噬细胞分化[J].2003,(1).
- [5]Gibbs JB.Anticancer drug targets: growth factors and growth factor signaling.[J].2000,105(1).
- [6]S, Messinetti,L, Giacomelli,G, Fabrizio,etc.CD44v6 and Nm23-H1 protein expression related to clinico pathological parameters in colorectal cancer.[J].1900,74(1).
- [7]吕翠仙,樊廷俊,胡国斌,等.凋亡诱导因子与细胞凋亡[J].2003,(10).
- [8].细胞内游离钙浓度在胃癌细胞凋亡过程中的动态变化及发生机制[J].1999,(5).
- [9]O'Rourke Michelle.Determining the Efficacy of Glucosamine and Chondroitin for Osteoarthritis[J].2001.
- [10]Florent, Richy,Olivier, Bruyere,Olivier, Ethgen,etc.Structural and symptomatic efficacy of glucosamine and chondroitin in knee osteoarthritis: a comprehensive meta-analysis.[J].2003,163.
- [11]Agnantis NJ,Makrydimas G,Zagorianakou N,etc.CD44 family and gynaecological cancer.[J].2003,17(6).
- [12]María J, Maté,Miguel, Ortiz-Lombardía,Brigitte, Boitel,etc.The crystal structure of the mouse apoptosis-inducing factor AIF.[J].2002,9(6).
- [13]Dwarakanath BS,Khaitan D,Ravindranath T.2-deoxy-D-glucose enhances the cytotoxicity of topoisomerase inhibitors in human tumor cell lines.[J].2004,3(9).
- [14]Marja, J??ttel?.Programmed cell death: many ways for cells to die decently.[J].2002,34.
- [15]Ursula Günthert,Martin Hofmann,Wolfgang Rudy,etc.A new variant of glycoprotein CD44 confers metastatic potential to rat carcinoma cells[J].1991,65(1).
- [16]罗宣干.5-氟脲嘧啶的D-氨基葡萄糖衍生物的合成及其抗肿瘤活性的研究[J].1996,(9).
- [17]Yang, W.,Toffa, S. E.,Lohn, J. W.,etc.Malignant ascites increases the antioxidant ability of human ovarian (SKOV-3) and gastric adenocarcinoma (KATO-III) cells[J].2005,96(2).
- [18]Delafuente JC.Glucosamine in the treatment of osteoarthritis.[J].2000,26(1).
- [19]Da-Qiang, Li,Zhi-Biao, Wang,Jin, Bai,etc.Effects of mifepristone on proliferation of human gastric adenocarcinoma cell line SGC-7901 in vitro.[J].2004,10.
- [20]Karrie Wong,Umayal Rubenthiran,Serge Jothy.Motility of colon cancer cells: modulation by CD44 isoform expression[J].2003,75(2).
- [21]N, Joza,S A, Susin,E, Daugas,etc.Essential role of the mitochondrial apoptosis-inducing factor in programmed cell death.[J].2001,410(6828).