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IL-4 enhances antitumor effect of IL-2 induced A-NK Cells on human colon carcinoma
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 9
- DOI:
- 10.3969/j.issn.1009-3079.2003.09.029
- Key Word:
- No keyword available
Abstract： 目的:用IL-4联合IL-2共同诱导A-NK细胞,研究A-NK细胞体外细胞毒性及对直肠肿瘤生长的抑制作用.方法:用基因重组的IL-2和IL-4联合活化A-NK细胞,用LDH-L释放法测定效应细胞的细胞活性,同时观察A-NK细胞对人直肠癌细胞在裸鼠体内的生长抑制作用.结果:IL-2单独或IL-2联合IL-4均可以诱导A-NK细胞,但二者联合效果更好,通过LDH-L测定活化的A-NK细胞可在体外杀伤K562,Anip973和CC95细胞(39.000±9.16vs77.68±12.80,43.10±10.05 vs 80.02±13.74,42.14±9.72vs79.10±2.65,P＜0.01),抑制人直肠癌肿瘤在裸鼠体内的生长(1.04±0.15 vs 0.62±0.16,P＜0.01),提示A-NK细胞膜表面存在IL-4受体的表达.结论:IL-4能够增强IL-2诱发的A-NK细胞抗肿瘤活性,此方法在将来肿瘤临床治疗中具有潜在的应用价值.
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