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Collaborative effect of tumor-derived HSP70 and IL-2 on mouse hepatocellular carcinoma cell line implanted tumor
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 7
- DOI:
- 10.3969/j.issn.1009-3079.2002.07.003
- Key Word:
- No keyword available
Abstract： 目的:研究肿瘤热休克蛋白70联合应用IL-2对小鼠肝癌的治疗作用,为两种免疫制剂联合应用治疗人类恶性肿瘤提供参考依据.方法:应用细胞培养、蛋白提纯、电泳、Western-blot、毛细管电泳、动物实验等方法.小鼠分别用HSP70 10μg、5 μg和IL-2 50kU、100kU、2kU(维持量)按设计的方案进行治疗试验.结果:单纯应用或联合应用HSP70和IL-2均能明显地延缓小鼠移植肝癌的生长并能不同程度地延长生存期,与对照组相比(除IL-250kU组外)差异显著(P＜0.025-0.05).但获长期存活(＞90d)者仅见于接受HSP70 10 μ g治疗组,40%(2/5)的HSP70 10 μ g组及HSP70 10 μ g联合应用IL-2 50kU组小鼠,和60%(3/5)的HSP70 10 μg联合应用IL-2 100kU组小鼠,获长期生存的小鼠其肿瘤最终全部消退.与无长期生存小鼠的其他组相比差异十分显著(P＜0.025-0.05).IL-2 100kU与HSP70 5 μ g的作用相当.结论:肿瘤来源的HSP70较IL-2具有更强的治疗作用,在联合应用实验的治疗组中HSP70对延缓和消除肿瘤起主要作用.更重要的是,合适剂量的HSP70与IL-2联合应用(本研究中HSP70 10 μg与IL-2 100kU)能明显地提高对荷瘤小鼠的治疗作用.
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