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Alterations of nitric oxide in apoptosis of esophageal carcinoma cells induced by arsenite
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 10
- DOI:
- 10.3969/j.issn.1009-3079.2000.10.006
- Key Word:
- 食管肿瘤;细胞凋亡;氧化砷;一氧化氮;免疫组织化学;显微镜检查/电子
Abstract： 目的一氧化氮NO已被认为是生理和病理状态下一种重要生物分子.以氧化砷(As2O3)诱导食管癌细胞(SHEEC1)凋亡过程中检测细胞NO,NO合成酶(NOS)和一氧化氮合成酶mRNA(NOSmRNA),探索NO和细胞凋亡的关系.方法用As2O3(5μmol·L-1和10 μmol·L-1)诱导SHEEC1凋亡,在加药后2,4,8,16,24 h取样检查,用分光光密度法检测细胞培养液的NO含量;用免疫组化检测诱导型NO合成酶(NOSⅡ);分子原位杂交(ISH)检测NOSmRNA;实验终点细胞作透射电镜(TEM)检查细胞凋亡.结果在As2O3诱导下,SHEEC1从NO基础量(0.68×10-2μmol·L-1)逐渐增加,至加药后16 h达最高(2.38×10-2μmol·L-1);NOSⅡ在加药后4 h～16h呈阳性反应;NOSmRNA杂交信号位于胞质,4 h杂交点小而少,以后增加明显.24 h,TEM可见SHEEC1出现细胞凋亡.结论As2O3可诱导SHEEC1释放NO和细胞凋亡,实验开始至16 h NO量不断增加.NOSⅡ表达和NOSmRNA转录上调.提出NO可能是As2O3诱导细胞凋亡的介导和作用因子.
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