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A case-control study on the relationship between Helicobacter pylori expressed cytotoxin associated gene A (CagA) and gastroduodenal ulcer

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Author:: No author available

Journal Title:: WORLD CHINESE JOURNAL OF DIGESTOLOGY

Issue:: 7

DOI:: 10.3969/j.issn.1009-3079.2000.07.003

Key Word:: 螺杆菌,幽门;胃溃疡;十二指肠溃疡;细胞毒素类;病例对照研究;胃镜检查

Abstract：目的本文从流行病学角度研究西安地区幽门螺杆菌细胞毒素相关蛋白A(CagA)阳性Hp菌株感染与胃十二指肠溃疡发病的关系.方法采用病例对照研究方法,病例组与对照组的配比比例为1:2.病例组为本院消化科自1997-10/1998-10连续住院的患者,经内镜检查及病理检查证实为活动期胃溃疡和(或)十二指肠溃疡.对照组分为两组,对照1组为同期住院的本科及其他科住院的非胃十二指肠疾病患者,对照2组为同期普查健康人群.病例组与对照组的配比因素为性别、民族、年龄(相差5岁以内).采用问卷调查,内容涉及与胃十二指肠溃疡发病可能有关的32个因素,对所有因素进行量化,应用SAS软件进行处理.CagA阳性Hp感染的检测结果分析采用X2检验.对所调查因素首先进行1:1及1:2单因素条件Logistic回归分析,有显著性意义的因素,计算近似相对危险度(odds ratio,OR)及其95%的可信区间(confidenceinterval,CI).两因素协同作用采用分层分析方法.对单因素分析时作用较大及调查结果较准确的因素,用多因素条件Logistic回归分析.血清中抗CagA抗体的检测采用斑点金免疫渗滤法(DIGFA).结果①在胃溃疡组与对照1组及对照2组的配对研究中,胃溃疡组的CagA阳性Hp感染率显著高于两个对照组,OR分别为5.0与6.5,P分别＜0.05及＜0.01.②在十二指肠溃疡组与对照1组及对照2组的配对研究中,十二指肠溃疡组的CagA阳性Hp感染率显著高于两个对照组,OR分别为9.33与11.00,P分别＜0.05及＜0.01.③1:1及1:2单因素及多因素Logistic回归分析表明,CagA阳性Hp感染、吸烟、饮酒、食酸辣食物、生活不规律、精神因素等因素与胃十二指肠溃疡发病呈正相关,而按时进餐与胃十二指肠溃疡发病呈负相关.CagA阳性Hp感染与胃溃疡及十二指肠溃疡的发生具有密切的相关性(OR=16.61-84.49).④双因素分层分析中,吸烟、饮酒、生活不规律、精神因素、食酸辣食物等因素与CagA阳性Hp感染之间作用相互独立,但在胃十二指肠溃疡病的发生中具有协同作用.结论消化性溃疡的发生是众多因素共同作用的结果,CagA阳性Hp菌株感染是引起胃溃疡及十二指肠溃疡多因素病因学中一个非常重要的、危险的因素.重视CagA阳性Hp菌株的研究,对预防和治疗消化性溃疡具有重要的临床意义和社会意义.

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