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Construction of pcDNA3/p16 plasmid and its inhibitory role in the proliferation of hepatoma cell line
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 1
- DOI:
- 10.3969/j.issn.1009-3079.2000.01.014
- Key Word:
- 肝肿瘤;p16基因;基因治疗;脂质体;转染
Abstract： 目的构建pcDNA3/p16真核表达质粒并了解其对肝癌细胞BEL-7404生长的抑制作用.方法将p16 cDNA亚克隆至pcDNA3真核表达载体上,并经脂质体介导转染至BEL-7404细胞中.应用MTT法和流式细胞仪分析转染细胞生长情况和细胞周期.结果重组pcDNA3/p16表达质粒构建成功.经pcDNA3/p16转染的BEL-7404细胞生长速度受到明显抑制,且细胞多停滞于G0/G1期.结论重组pcDNA3/p16质粒能在BEL-7404细胞内表达,且能抑制BEL-7404细胞的生长.
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