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Protective Effect of MitoKATP Opener on Energy Metabolism during Ischemic and Reperfusion in Isolated Working Rat Heart
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- Author:
- No author available
- Journal Title:
- JOURNAL OF NAVAL GENERAL HOSPITAL OF PLA
- Issue:
- 1
- DOI:
- 10.3969/j.issn.1009-3427.2006.01.001
- Key Word:
- 心肌缺血再灌注损伤;细胞色素氧化酶;H+-三磷酸腺苷;二氮嗪
Abstract： 目的观察线粒体内膜三磷酸腺苷敏感钾离子通道开放剂二氮嗪加入停搏液内对缺血再灌注大鼠心肌能量代谢的保护作用.方法 50只大鼠随机分为五组,每组10只,取离体心脏;A组取正常心肌,其余制备离体工作心脏灌注模型;B组停灌30 min,复灌60 min;其余三组分别灌注不同的停搏液,C组灌注停搏液,D组灌注含二氮嗪的停搏液,E组灌注含二氮嗪停搏液之前10 min给予格列苯脲,各组复灌30 min后进行工作模式心脏灌注,监测心率、左室收缩峰压和舒张末压.实验终了取心肌,提取线粒体,测定细胞色素氧化酶活性、线粒体H+-三磷酸腺苷酶活性;高效液相法测定心肌含量.结果缺血再灌注心肌线粒体细胞色素氧化酶活性和线粒体H+-三磷酸腺苷酶活性降低(P＜0.05);而二氮嗪处理组相应指标显著改善(P＜0.01).结论含二氮嗪停搏液能够改善缺血再灌注心肌功能,能量代谢恢复.
- [1]B, O'Rourke.Myocardial K(ATP) channels in preconditioning.[J].2000,87(10).
- [2]Y, Toyoda,S, Levitsky,J D, McCully.Opening of mitochondrial ATP-sensitive potassium channels enhances cardioplegic protection.[J].2001,71(4).
- [3]Korge P,Honda HM,Weiss JN.Regulation of the mitochondrial permeability transition by matrix Ca(2+) and voltage during anoxia/reoxygenation.[J].2001,280(3).
- [4]S L, Maskal,N M, Cohen,P W, Hsia,etc.Hyperpolarized cardiac arrest with a potassium-channel opener, aprikalim.[J].1995,110.
- [5]G J, Gross,R M, Fryer.Sarcolemmal versus mitochondrial ATP-sensitive K+ channels and myocardial preconditioning.[J].1999,84(9).
- [6]J R, Neely,H, Liebermeister,E J, Battersby,etc.Effect of pressure development on oxygen consumption by isolated rat heart.[J].1967,212(4).