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Application of laser capture microdissection in the molecular pathological diagnosis of and pathogenesis research on infectious skin diseases
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- Author:
- No author available
- Journal Title:
- International Journal of Dermatology and Venereology
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1673-4173.2014.03.018
- Key Word:
- 皮肤病,感染性;分子病理学;激光捕获微切割;Skin diseases,infectious;Molecular pathology;Laser capture microdissection
Abstract： Laser capture microdissection (LCM) technology allows the harvesting of specific cell populations and even single cells by cutting a section of complex and heterogeneous tissue.The DNA,RNA and protein within the cells of interest can be analyzed by using LCM in conjunction with molecular biology techniques,which may improve pathologically diagnostic capability and promote pathogenesis research.In combination with PCR,LCM may be used to assess clonality in cutaneous lymphocytic infiltrates,explore infection pathogenesis,and detect common pathogenic microorganisms such as herpes simplex virus (HSV),varicella zoster virus (VZV),Mycobacterium tuberculosis in skin specimens from patients with infectious dermatoses.
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