Application value of glutamate chemical exchange saturation transfer imaging in radiation-induced brain injury rat
- Author:
- No author available
- Journal Title:
- Chinese Journal of Magnetic Resonance Imaging
- Issue:
- 12
- DOI:
- 10.12015/issn.1674-8034.2023.12.014
- Key Word:
- 放射性脑损伤;认知障碍;放射治疗;化学交换饱和转移成像;磁共振成像;radiation brain injury;cognitive impairment;radiation therapy;chemical exchange saturation transfer;magnetic resonance imaging
Abstract： Objective:To investigate the value of 7.0 T magnetic resonance glutamate chemical exchange saturation transfer(GluCEST)imaging for assessing hippocampal glutamate changes in a rat model of radiation brain injury.Materials and Methods:The experimental rats were randomly divided into control group(n=9)and radiation-induced brain injury(RBI)group(n=9).Rats in the RBI group were irradiated using an X-ray irradiator and subjected to a water maze experiment 4 weeks later.Chemical exchange saturation transfer(CEST)sequences were collected using 7.0 T small animal magnetic resonance and hippocampal glutamate concentrations were measured by high-performance liquid chromatography(HPLC).The t-tests were performed for differences in GluCEST and glutamate concentrations in the hippocampal brain regions between the two groups.Pearson correlation analyses were performed for the relationship between GluCEST and HPLC and between GluCEST and water maze.Results:Compared to control group,rats in the RBI group had impaired cognitive function and reduced hippocampal GluCEST(P=0.008)after 4 weeks,and reduced glutamate levels were confirmed by HPLC measurements(P=0.003).Correlation analysis showed a positive correlation between glutamate concentration by HPLC and GluCEST(r=0.71,P＜0.001),and spatial memory also showed a consistent positive correlation with hippocampal GluCEST(r=0.50,P=0.034).Conclusions:GluCEST imaging visualizes and evaluates hippocampal glutamate changes of RBI rats and might be used as an imaging biomarker for metabolic changes in vivo,which might be useful for the monitoring and intervention of concomitant radiation damage after radiotherapy.
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