Profiling of Membrane Lipids of Arabidopsis Roots during Catechin Treatment
- Author:
- No author available
- Journal Title:
- Plant Diversity and Resources
- Issue:
- 4
- DOI:
- 10.3724/SP.J.1143.2012.12005
- Key Word:
- 植物毒素;脂类组学;儿茶素;拟南芥;Phytotoxin;Lipidomics;Catechin;Arabidopsis
Abstract： Catechin is a kind of phytotoxin and can kill plant cells in an hour.It can be developed as herbicide to kill weeds due to its strong phytotoxic activity.The main effect of this chemical is to trigger the death of the root system.To understand thc rcsponse of root cell membrane lipids to catechin stress,we used the lipidomics approach to study the profiles of Arabidopsis root lipids molecules under catechin treatment.The changes of molecular species in membrane lipids,content of membrane lipids,double bond index (DBI) and acyl chain carbon number of the fatty acid were examined in Wild type (WS) and PLDδ deficient mutant (PLDδ-KO) during catechin treatment.The results indicated that after 90 min treatment with catechin,the lipid contents of digalactosyldiacylglycerol (DGDG),monogalactosyldiacylglycerol (MGDG),phosphatidylglycerol ( PG),phosphatidylcholine ( PC),and phosphatidylinositol (PI) decreased both in WS and PLDδ-KO roots,lipid contents of phosphatidylethanolamine (PE) and phosphatidylinositol (PS) decreased in WS roots,but increase in PLDδ-KO roots,lipid contents of (phosphatidic acid)PA increased at the begin of treatment and declined to the level of control in WS roots.The ratio of the two major lipids in roots,PC and PE,declined significantly in PLDδ-KO plants,the acyl carbon number of PS in WS plants increased.The results suggested that PLDδ-KO is more sensitive than WS during catechin treatment,and suppression of PLDδ exacerbated membrane damage induced by catechin.
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