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Expression of sperm protein 32/OY-TES-1 in retinoblastoma

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Author:
No author available
Journal Title:
CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
Issue:
1
DOI:
10.3760/cma.j.issn.1005-1015.2011.01.004
Key Word:
视网膜母细胞瘤/病理生理学;基因表达;抗原,肿瘤;Retinoblastoma/pathophysiology;Gene expression;Antigens,neoplasm

Abstract: Objective To observe the expression of sperm protein (SP) 32/OY-TES-1 in retinoblastoma (RB). Methods Thirty paraffin specimens of pathologically confirmed RB eyeballs were investigated in this study. The SP32/OY-TES-1 mRNA expression of 15 RB tissues, 12 non-tumor retinal tissues and 22 normal eye tissues were detected by reverse transcription polymerase chain reaction (RT-PCR). The SP32/OY-TES-1 protein expression of 30 RB tissues and 24 normal retinal tissues were examined by immunohistochemistry. The relationship between SP32/OY-TES-1 mRNA, protein expression and age, gender, tumor size, tumor differentiation, clinical stage of RB children were analyzed. Results The expression rate of SP32/OY-TES-l mRNA in RB tissues, non-tumor retinal tissues and normal eye tissues were 86.7%, 16. 7% and 36. 4% respectively. Compared the expression rate of SP32/OY-TES-1mRNA in different gender (χ2 = 0.744 ), age (χ2 = 1.178 ), clinical stage (χ2 = 1. 188 ), tumor size (χ2 =0. 216) ,tumor differentiation (χ2 = 1. 885),the differences were not statistically significant (P>0. 05). The expression rate of SP32/OY-TES-1 protein in RB tissues was 73.3% and no expression in normal retinal tissues. Compared the expression rate of SP32/OY-TES-1 protein in different gender (χ2= 1.000), age with ≤ two years and > two years (χ2 = 0. 403), tumor size (χ2 = 2. 274), tumor differentiation (χ2=0. 138), the differences were not statistically significant (P>0.05) ;but in clinical stage (χ2 =6. 193) ,with or without optic nerve infiltration (χ2= 4. 535), the differences were statistically significant (P<0.05).Conclusions SP32/OY-TES-1 is highly expressed in RB. The expression rate of SP32/OY-TES-1 is related to optic nerve infiltration and clinical stage of RB.

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