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Proliferation and characters of differentiation of retinal stem cells after cryopreservation and anabiosis in rat
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
- Issue:
- 2
- DOI:
- 10.3760/j.issn:1005-1015.2007.02.004
- Key Word:
- 干细胞/细胞学;干细胞/病理学
Abstract： 目的 研究视网膜干细胞冻存复苏后的成活率及再培养后的增生分化特性.方法 对胎龄17 d Long Evans的大鼠眼视网膜干细胞进行分离与体外培养.利用无血清培养基短期体外培养大鼠胚胎来源的视网膜干细胞,将传代三代后的视网膜干细胞以冻存液[体积分数为80%的改良基础培养基(DMEM)/F12,10%牛血清白蛋白(BSA),10%二甲基亚砜(DMSO),碱性成纤维细胞生长因子(bFGF)20ng/ml]于液氮中冻存,于1、2、4、8、12、16周分别复苏,计数活细胞比例进行再培养,并进一步诱导分化,采用免疫细胞荧光化学方法检测视网膜干细胞的增生及分化特性.结果 不同的冻存时间对冻存后细胞存活率的影响无显著差异(P＞0.05),再培养生长良好,具有视网膜干细胞特异性标志,并可进一步诱导分化为视网膜各种类型的细胞.结论 大鼠视网膜干细胞的冻存复苏并不影响其原有的增生及分化特性.
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