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Infected endophthalmitis diagnosed by multiplex polymerase chain reaction
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
- Issue:
- 2
- DOI:
- 10.3760/j.issn:1005-1015.2004.02.005
- Key Word:
- 聚合酶链反应;眼内炎/诊断;眼内炎/微生物学;细菌;真菌
Abstract： 目的探讨多重聚合酶链反应(MPCR)快速诊断细菌性、真菌性眼内炎及混合感染的价值. 方法 MPCR法检测常见致病性细菌和真菌菌株,并检测38例患者41只眼(双眼3例,单眼35例)眼内液标本,与细菌和真菌培养结果比较. 结果 MPCR法5 h即可检测出标本中微量细菌和真菌,41份标本MPCR检测阳性34份(82.9%),其中细菌阳性26份,真菌阳性6份,细菌和真菌均为阳性2份,MPCR阳性率明显高于细菌或真菌培养(χ2＝9.60,P＜0.05). 结论 MPCR速度快、敏感性和特异性高,有助于细菌性和真菌性眼内炎的快速明确诊断.
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