You Position: Home > Paper
Effect of leukocyte on early diabetic retinopathy
( views:392, downloads:107 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
- Issue:
- 6
- DOI:
- 10.3760/j.issn:1005-1015.2003.06.005
- Key Word:
- 糖尿病视网膜病;白细胞;链脲佐菌素;疾病模型,动物
Abstract： 目的探讨白细胞在早期糖尿病大鼠视网膜毛细血管中粘附、堆积及其与视网膜微血管形态学变化的关系. 方法健康成年雄性Wistar大鼠90只,随机分成正常对照组和链脲佐菌素(streptozotocin, STZ)诱导的糖尿病组各45只(3、7、14 d组各5只,30、90、180 d组各10只).取大鼠右眼制备视网膜消化铺片,进行形态学观察及白细胞共有抗原(leukocyte common antigen, CD45)单克隆抗体免疫组织化学研究. 结果正常对照组视网膜毛细血管中有少许CD45阳性细胞;糖尿病发生3 d后,CD45的表达明显增加,病程第14 d达高峰,以后基本稳定.病程90 d时,视网膜毛细血管已出现节段性膨大、囊样扩张及闭锁等形态学改变;病程180 d时,上述病变进一步加重. 结论白细胞粘附发生在糖尿病视网膜病变(diabetic retinopathy, DR)的早期阶段,是DR在微血管水平病理变化的开端.白细胞粘附可能作为视网膜微血管形态学改变的基础,在DR的发生发展中起着重要作用.
- [1]G A, Lutty,J, Cao,D S, McLeod.Relationship of polymorphonuclear leukocytes to capillary dropout in the human diabetic choroid.[J].1997,151(3).
- [2]Tang, S.,Scheiffarth, O. F.,Thurau, S. R.,etc.Cells of the Immune System and Their Cytokines in Epiretinal Membranes and in the Vitreous of Patients with Proliferative Diabetic Retinopathy[J].1993,25(3).
- [3]KUWABARA TOICHIRO.Studies of Retinal Vascular Patterns[J].1960.
- [4]L W, Kelly,C A, Barden,J S, Tiedeman,etc.Alterations in viscosity and filterability of whole blood and blood cell subpopulations in diabetic cats.[J].1993,56(3).
- [5]S F, Freedman,D L, Hatchell.Enhanced superoxide radical production by stimulated polymorphonuclear leukocytes in a cat model of diabetes.[J].1992,55(5).
- [6]Miyamoto K,Tsujikawa A,Ogura Y,etc.In vivo demonstration of increased leukocyte entrapment in retinal microcirculation of diabetic rats.[J].1998,39(11).
- [7]ShiboTang,KimChiLe-Ruppert.Activated T lymphocytes in epiretinal membranes from eyes of patients with proliferative diabetic retinopathy[J].1995,233(1).
- [8]K, Miyamoto,Y, Ogura.Pathogenetic potential of leukocytes in diabetic retinopathy.[J].1999,14.
- [9]E M, Kohner.Diabetic retinopathy.[J].1989,45(1).