Effects of glycation products on growth and cytosolic free calcium in bovine retinal capillary pericytes
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
- Issue:
- 3
- DOI:
- 10.3760/j.issn:1005-1015.2000.03.012
- Key Word:
- 糖基化终产物,高级;视网膜/药物作用;胸腺嘧啶核苷酸类;钙/分析;增生
Abstract： 目的 探讨糖基化产物对牛视网膜周细胞(pericyte,PC)增生和胞浆[Ca2+]i水平的影响.方法 采用细胞计数结合噻唑蓝比色测定,氚标胸腺嘧啶核苷(3H-thymidine,3H-TdR)掺入和钙荧光探剂(Fura-2Acetoxymethyl ester,Fura-2AM),研究PC在牛血清白蛋白早期糖基化产物(early glycation pro-ducts of bovine serum albumin,EG-BSA)和牛血清白蛋白糖基化终产物(advanced glycation end products of bovine serum albumin,AGE-BSA)培养下,PC增生数、DNA合成量及胞浆[Ca2+]i水平的改变.结果 培养4 d后,细胞计数法:EG-BSA和AGE-BSA组PC数分别为17.87±2.36,14.77±3.72,较其对照组(20.54±0.82,20.31±0.93)减少13.00%和27.00%(P＜0.01);MTT法:EG-BSA和AGE-BSA组分别为0.4619±0.0946,0.3884±0.1013,较其对照组(0.5236±0.0539,0.5227±0.0519)减少12.00%和25.70%(P＜0.01);3H-TdR掺入量:EG-BSA和AGE-BSA组分别为39450.16±8870.68,33667.85±10581.70,较其对照组(56373.63±2317.97,56542.04±1961.23)减少30.00%和40.46%(P＜0.01);胞浆[Ca2+]i水平:EG-BSA和AGE-BSA组分别为(129.55±30.41) nmol/L,(179.71±56.69) nmol/L,较其对照组[(79.70±6.94) nmol/L,(83.96±6.39) nmol/L]增高163.00%和214.00%(P＜0.01).结论 EG-BSA和AGE-BSA能不同程度地抑制视网膜微血管PC增生和DNA合成,并使胞浆[Ca2+]i水平增高,尤以AGE-BSA为著.
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