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Comparison of thyroid function tests results determined by two kinds of immunoassay in uncomplicated pregnant women

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Author:
No author available
Journal Title:
Chinese Journal of Perinatal Medicine
Issue:
7
DOI:
10.3760/cma.j.issn.1007-9408.2012.07.002
Key Word:
妊娠;促甲状腺素;甲状腺原氨酸类;试剂盒,诊断;参考值;Pregnancy;Thyrotropin;Thyronines;Reagent kits,diagnostic;Reference values

Abstract: Objective To compare thyroid function test (TFT) results and reference range of normal pregnant women in different gestational stages between two kinds of automation chemiluminescence immunoassay kit commonly used.Methods Serum of 693 normal pregnant women attended antenatal clinics in the International Peace Maternity & Child Health Hospital Affiliated to the Medical School of Shanghai Jiaotong University from Feb.17th to June 9th,2011 was collected,and were divided into five groups according to their gestational age,9-13,16-20,24-28,32-34 and 37-40 weeks.Thyroid stimulating hormone (TSH),free thyroxine (FT4) and free triiodothyronine (FT3) levels were determined by 2 kinds of detection reagents:Abbott Architect I2000 and Roche Cobas Eleesys 600.According to National Academy of Clinical Biochemistry (NACB),the reference range of the TFT indexes was calculated.The results were analyzed by one-way ANOVA,t-test and Spearman relation analysis.Results During pregnancy,TSH level at different stage of pregnancy detected by the two reagents showed linear correlation and the changing pattern was also consistent (r =0.833-0.973,P =0.000).In 9-13 gestational weeks group,TSH level were significantly lower than that of other groups; and in 37-40 gestational week group,it was higher than that of other groups (FAbbott =18.830,FRoche =21.012,all P=0.000).And TSH levels and its reference range determined by Roche detection reagent in each group were higher than those by Abbott detection reagent (|t| =3.002,4.948,6.353,4.636 and 4.391,P<0.01,respectively).In 9-13 gestational weekgroup,FT4 level was higher than that of other groups (F A b bott =17.230,FRoche =14.439,P=0.000).In 9-13 week group,FT4 level determined by Roche reagent was higher than that detected by Abbott reagent (|t| =4.964,P=0.000).While in 24-28 and 37-40 weeks group,they were lower (|t| =4.183,P=0.000; |t| =2.417,P=0.016).In 9-13 and 16-20 gestational weeks group,FT:3 levels,determined by both Abbott and Roche reagents,were higher than those of other groups (FAbbott =46.142,FRoche=68.149,P=0.000).The FT3 level in each group detected by Roche reagent were higher than that detected by Abbott reagent (|t| =31.762,26.411,28.877,22.710 and 22.736,P=0.000).Only in 9-13 weeks group,the TSH level was related to FT4 and FT3 level determined byboth reagents (Abbott:r=-0.319 and -0.361,P=0.000; Roche:r=-0.352 and -0.329,P=0.000).Conclusions TSH serum level is the most accurate indicator of thyroid function.The upper limit of the empirical TSH reference range during pregnancy recommended by America Thyroid Association might not be appropriate for Chinese.Different reference range for pregnant women should be established according to different reagents applied.

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