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Effect of placental growth factor-1 on primary cultured human umbilical vein endothelial cell proliferation and nitric oxide production
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PERINATAL MEDICINE
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1007-9408.2008.01.003
- Key Word:
- 妊娠蛋白质类;脐静脉;内皮细胞;一氧化氮;细胞增殖
Abstract： 目的 了解胎盘生长因子-1(placenta growth factor-1,PLGF-1)对原代培养人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVEC)增殖及一氧化氮(nitric oxide,NO)释放的影响.方法 取正常晚孕期剖宫产儿脐带,采用胰蛋白酶消化法,进行HUVEC原代培养.培养成功后,收集细胞,用免疫印迹法和RT-PCR法检测酪氨酸激酶受体(fms-like tyrosine kinase,Fit-1)蛋白和基因表达,继之用不同浓度PLGF-1处理细胞,然后在不同时间点收集细胞培养液,用硝酸盐还原酶法测细胞培养液上清中NO水平;用MTT比色法测细胞增殖水平.结果 Flt-1基因和蛋白在HUVEC中有表达.PLGF-1可显著诱导HUVEC释放NO,并呈剂量依赖关系.PLGF-1对HUVEC有增殖作用.结论 PLGF-1可刺激HUVEC增殖和诱导HUVEC释放NO.
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