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Inhibitory effects of vitamin E on expression of NF-κB and VCAM-1 in cultured human umbilical vascular endothelial cell induced by serum of pre-eclampsia

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Author:
No author available
Journal Title:
CHINESE JOURNAL OF PERINATAL MEDICINE
Issue:
6
DOI:
10.3760/cma.j.issn.1007-9408.2007.06.001
Key Word:
先兆子(癎);维生素E;NF-κB;脐静脉;内皮细胞;血管细胞黏附分子-1

Abstract: 目的 探讨子(癎)前期(pre-eclampsia,PE)血清诱导人脐静脉内皮细胞(human umbilical vascular endothelial cell,HUVEC)血管内皮细胞黏附分子-1(vascular cell adhesion molecules-1,VCAM-1)的表达、核因子-κB(nuclear factor kappa B,NF-κB)活性及维生素E(vitamin E,Vit E)对其的影响.方法 采用胰酶消化培养法培养正常妊娠HUVEC,传代后待细胞长满至70%~80%,加或不加Vit E作用30 min后分别加入正常妊娠及PE血清,培养2 h,Western印迹法测定细胞胞浆核因子κB抑制因子(inhibit I kappa B,I-κB)、胞核NF-κB p65含量;培养48 h,MTT测定细胞活力,流式细胞学测定细胞凋亡,酶联免疫法测定HUVEC和VCAM-1的表达.结果 PE血清培养的HUVEC胞浆I-κB含量明显低于对照组,胞核NF-κB p65含量、VCAM-1的表达、细胞凋亡率明显高于正常妊娠组(P<0.05),细胞活力明显低于对照组(P<0.05).加Vit E预处理后:PE组HUVEC胞浆I-κB含量明显增加;胞核NF-κB p65含量、VCAM-1的表达、细胞凋亡率明显下降(P<0.05),细胞活力明显增加(P<0.05). 结论 PE血清可促进HUVEC NF-κB活性及VCAM-1的表达,Vit E可抑制PE患者血清诱导HUVEC的NF-κB活性及VCAM-1的表达.

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