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Quantitative analysis of fetal DNA in maternal plasma
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PERINATAL MEDICINE
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1007-9408.2006.05.004
- Key Word:
- 妊娠;DNA;聚合酶链反应;产前诊断
Abstract： 目的 探讨应用荧光定量PCR技术检测孕妇血浆中胎儿DNA量,了解胎儿DNA和母体DNA随孕周增长的变化,为将来应用于非创性产前诊断做临床前期研究.方法 选择妊娠7～42周,B超确诊为单胎的孕妇58例,使用DNA试剂盒提取孕妇血浆中的胎儿DNA,用荧光定量PCR(FQ-PCR)技术测定血浆中β-actin基因和SRY基因的量.结果 58例正常孕妇,有37例男性胎儿,检出率为100%.胎儿DNA的量在早孕组为9.08拷贝/ml(3.5～12.8),中孕组为45.41拷贝/ml(14.38～76.5),晚孕组为300.95拷贝/ml(84～840).21例女性胎儿均未检出SRY基因.结论胎儿DNA随着孕周的增加而增长,母体血浆是进行无创性产前诊断的非常有价值的底物.
- [1]Aggeliki, Kolialexi,George Th, Tsangaris,Aristides, Antsaklis,etc.Rapid clearance of fetal cells from maternal circulation after delivery.[J].2004,1022.
- [2]S S Y, Ho,Z, Damayanti,W Y, Chua,etc.Non-invasive prenatal diagnosis of fetal gender using real-time polymerase chain reaction amplification of SRY in maternal plasma.[J].2004,33.
- [3]De Kok JB,Swinkels DW,Spencer K.Increased total cell-free DNA in the serum of pregnant women carrying a fetus affected by trisomy 21.[J].2003,23(7).
- [4]Y. M. Dennis Lo,Mark S.C. Tein,Tze K. Lau,etc.Quantitative Analysis of Fetal DNA in Maternal Plasma and Serum: Implications for Noninvasive Prenatal Diagnosis[J].1998,62(4).
- [5]Y M, Lo,T K, Lau,J, Zhang,etc.Increased fetal DNA concentrations in the plasma of pregnant women carrying fetuses with trisomy 21.[J].1999,45.
- [6]Hjelm NM,Lo YM,Lau TK,etc.Maternal plasma fetal DNA as a marker for preterm labour (letter)[J].1998,352(9144).
- [7]Sinclair, A.H.,Berta, P..A gene from the human sex-determining region encodes a protein with homology to a conserved DNA...[J].1990,346(6281).
- [8]Bohmer RM,Cha D,Johnson KL,etc.A simple and sensitive erythroblast scoring system to identify fetal cells in maternal blood.[J].2003,23(1).
- [9]Y M, Lo,T N, Leung,M S, Tein,etc.Quantitative abnormalities of fetal DNA in maternal serum in preeclampsia.[J].1999,45.
- [10]Zolotukhina TV,Shilova NV,Voskoboeva EY.Analysis of cell-free fetal DNA in plasma and serum of pregnant women[J].2005,53(3).
- [11]Y. M. Dennis Lo,Jun Zhang,Tse N. Leung,etc.Rapid Clearance of Fetal DNA from Maternal Plasma[J].1999,64(1).
- [12]Agnieszka, Orzińska,Katarzyna, Guz,Ewa, Brojer,etc.[Examination of the RHD gene by non-invasive method in plasma of alloimmunized mother allowed to abandon invasive procedures and to deliver a healthy child].[J].2004,75.
- [13]Cotter AM,O'leary JJ,Daly SF,etc.Increased fetal RhD gene in the maternal circulation in early pregnancy is associated with an increased risk of pre-eclampsia.[J].2005,112(5).
- [14]Hiromi Hamada,Tadao Arinami,Takeshi Kubo,etc.Fetal nucleated cells in maternal peripheral blood: frequency and relationship to gestational age[J].1993,91(5).
- [15]Bischoff FZ,Lewis DE,Simpson JL.Cell-free fetal DNA in maternal blood: kinetics, source and structure.[J].2005,11(1).
- [16]Lo YM,Corbetta N,Sargent IL,etc.Presence of fetal DNA in maternal plasma and serum.[J].1997,350(9076).
- [17]Viora E,Ferrari M,Pagliano M,etc.Fetal DNA detection in maternal plasma throughout gestation.[J].2005,117(2).
- [18]Choe J,Kim KC,Choi YM,etc.Fetal gender determination and Bcll polymorphism using nucleated erythrocytes in maternal blood[J].2005,53(3).
- [19]周荣家,程汉华,余其兴.SRY基因与性别决定[J].1995,(5).