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Study of ultrastructure and expression of epidermal growth factor receptor in placenta in intrahepatic cholestasis of pregnancy
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PERINATAL MEDICINE
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1007-9408.2004.06.005
- Key Word:
- 妊娠并发症;胆汁淤积,肝内;胎儿生长受限;受体,表皮生长因子
Abstract： 目的观察妊娠肝内胆汁淤积症(intrahepatic cholestasis of pregnancy,ICP)胎盘超微结构病理改变和表皮生长因子受体(EGFR)的表达,研究胆汁酸对滋养细胞的毒性作用及其对胎儿生长发育的影响.方法透射电镜观察22例ICP患者胎盘组织,其中ICP合并胎儿生长受限(fetal growth restriction,FGR)7例、ICP未合并FGR 15例,应用免疫组化方法和逆转录-聚合酶链反应(RT-PCR)技术测定胎盘滋养细胞表皮生长因子受体(EGFR)的表达,并和15例正常晚期胎盘比较.结果电镜显示,ICP胎盘合体滋养细胞较对照组微绒毛减少、内质网扩张、线粒体肿胀或髓鞘样改变,核染色质异常分布.ICP合并FGR组上述病理改变更为明显,绒毛间质毛细血管减少,并出现大量胶原原纤维.ICP合并FGR组胎盘EGFR免疫组化的平均A值(0.239±0.017)和ICP未合并FGR组EGFR免疫组化的平均A值(0.253±0.015)均比对照组(0.384±0.016)低,差异具非常显著性(P＜0.01).ICP合并FGR组胎盘EGFR mRNA指数(0.230±0.048)和ICP未合并FGR组EGFR mRNA指数(0.231±0.042)表达均比对照组(0.460±0.051)低,差异具非常显著性(P＜0.01),ICP合并FGR组与ICP未合并FGR组之间胎盘EGFR的表达无显著性差异(P＞0.05).结论胆汁酸对胎盘滋养细胞有细胞毒性作用;ICP胎盘滋养细胞EGFR的表达降低,可能是长时间高浓度胆汁酸刺激所致.
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