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cDNA array in the establishment of a profile in differently expressed genes in placental tissue from cases of pregnancy induced hypertension
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PERINATAL MEDICINE
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1007-9408.2004.04.002
- Key Word:
- 妊娠并发症,心血管;高血压;胎盘;寡核苷酸序列分析
Abstract： 目的检测妊娠高血压综合征(妊高征)患者胎盘组织和正常妊娠胎盘组织差异表达基因谱. 方法 Trizol法抽提组织总RNA并纯化mRNA;应用cDNA阵列人类全基因表达谱分析芯片检测6例妊高征患者胎盘组织和5例正常妊娠者胎盘组织中差异表达的基因,扫描仪分析结果. 结果所检测的17 000个基因中,妊高征患者胎盘组织与正常妊娠者胎盘组织之间存在差异表达基因96个,表达上调基因数为78个,下调基因数为18个.初步分析发现,有85个已知功能基因,其中79个功能分类基因,大致分为癌基因和细胞周期蛋白、细胞骨架和运动蛋白、细胞分裂、离子通道和运输蛋白、细胞凋亡和应激反应蛋白、细胞受体、免疫、细胞信号和传递蛋白、转录和翻译系统、代谢、发育、细胞分化相关基因,另外有6个未分类基因、8个未知基因表达序列标签、3个cDNA片段. 结论妊高征发病的分子机制可能涉及了一些基因表达的变化.
- [1]周容,李维敏,曹泽毅.妊高征患者胎盘中一氧化氮合酶-mRNA转录强度的变化及意义[J].1998,(12).
- [2]ten-Kate LP,Lachmeijer AM,Dekker GA,etc.Polymorphisms in the tumor necrosis factor and lymphotoxin-alpha gene region and preeclampsia.[J].2001,98(4).
- [3]Kuwasako K,Ishiyama Y,Kitamura K,etc.Increased plasma proadrenomedullin N-terminal 20 peptide in patients with essential hypertension.[J].1999,36(Pt.5).
- [4]T, Nakayama,T, Ishikita,H, Matsuura,etc.[Effects of long-term treatment with prostacyclin on plasma adrenomedullin in patients with primary pulmonary hypertension].[J].2001,38.
- [5]Y, Makino,K, Shibata,I, Makino,etc.Expression of adrenomedullin in feto-placental circulation of human normotensive pregnant women and pregnancy-induced hypertensive women.[J].1999,140.
- [6]Morrish DW,Davidge ST,Jerat S,etc.Effect of adrenomedullin on placental arteries in normal and preeclamptic pregnancies.[J].2001,37(2).
- [7]Jahn O,Eckart K,Brauns O,etc.The binding protein of corticotropin-releasing factor: ligand-binding site and subunit structure.[J].2002,99(19).
- [8]Matsumoto K,Ogihara T,Yo Y,etc.Prevention of renal damage by angiotensin II blockade, accompanied by increased renal hepatocyte growth factor in experimental hypertensive rats.[J].1999,34(2).
- [9]Wang Y,Walsh SW.Placental mitochondria as a source of oxidative stress in pre-eclampsia.[J].1998,19(8).