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Fetal Nucleated Red Blood Cell in Maternal Peripheral Blood: Frequency and Relationship with Gestational Age
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PERINATAL MEDICINE
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1007-9408.2000.04.002
- Key Word:
- 妊娠;红细胞;产前诊断;聚合酶链反应
Abstract： 目的 探讨用检测孕妇外周血中的胎儿有核红细胞(NRBC)进行无创性产前诊断的最佳时间.方法 对44名孕龄6～40周的孕妇外周血进行不连续密度梯度离心,将分离后的细胞进行制片,显微镜下行有核红细胞计数,然后用显微操作法一一获取,进行Y染色体特异性DYZ1基因的聚合酶链反应(PCR),以确定其胎儿来源.结果 44 名孕妇中有17例其外周血中检出有NRBC,分布于妊娠第9～26周,其中以妊娠第11～20周检出率最高,达76.5%(13/17).NRBC检出数量从5个/7 ml～30个/7 ml不等.PCR扩增Y染色体特异序列结果:7例扩增出特异性片段,另外10例未扩增出,所得结果与胎儿实际性别相吻合.结论 用胎儿有核红细胞行无创性产前诊断的最佳时间为妊娠第11～ 20周.
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