Smac/DIABLO and cytochrome c mRNA levels in liver tissue of rats with acute hepatic failure treated by microencapsulated hepatocytes
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1674-2397.2009.06.006
- Key Word:
- 细胞色素c类;第二个线粒体衍生的半胱氨酸蛋白酶激活剂;肝细胞移植;微囊化肝细胞;Cytochromes c;Second mitochondria-derived activator of caspase;Hepatocyte transplantation;Microencapsulated hepatocyte
Abstract： Objective To investigate Smac/DIABLO and cytochrome c(cyt-c)mRNA levels in liver tissue of rats with acute hepatic failure treated by microencapsulated hepatocyte.Methods Acute hepatic failure were induced by intraperitoneal injection of D-galactosamine in rats.and the rats were treated with microencapsulated hepatocytes,free hepatocytes and physical saline(contr01),respectively.Smac/DIABLO and cyt-c mRNA in liver tissue was detected by RT-PCR and the mRNA expression levels among three groups were compared.Results Smac/DIABLO and cyt-c mRNA levels in liver tissues of rats with acute hepatic failure were higher than those of normal rata(F=4.345,14.821,47.565,42.178 and 62.961,P<0.05).The peak values of Smac/DIABLO and cyt-C mRNA expressions in free hepatocytes and control groups were at 48 h.while that in microencapsulated hepatoeytes group was at 24 h.Conclusion Smac/DIABLO and cyt-c mRNA expression is an indicator of apoptosis of hepatocytes.
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