Enzyme-linked immunospot assay in diagnosis of Mycobacterium tuberculosis infection for AIDS patients
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1674-2397.2008.04.003
- Key Word:
- 获得性免疫缺陷综合征;分枝杆菌,结核;诊断;结核菌素试验;酶联免疫斑点技术;Acquired immunodeficiency syndrome;Mycobacterium tuberculosis;Diagnosis;Tuberculin test;Enzyme-linked immunospot assay
Abstract： Objective To evaluate the application of enzyme-linked immunospot assay (T SPOT) in diagnosis of Mycobacterium tuberculosis (MTB) infection for AIDS patients. Methods The rapid T SPOT assay was employed to detect ESAT-6 and CFP-10 specific T cells in blood samples from 35 AIDS patients with active tuberculosis. The results were compared with those of PPD skin test. Results The positive-MTB rate with T SPOT assay in all patients (n = 35 ) was 65.71%, that in patients with CD4 < 200/μ (n = 21) was 66.67%, in patients with CD4 > 200/μl (n = 10) was 60.0% , in patients with anti-tuberculosis treatment < 2 months ( n = 15 ) was 83.33% and in patients with treatment > 2 months ( n = 6 ) was 25.00%. The positive rates of PPD skin test were 24.24%, 25.00%, 33.33%, 9.09% and 25.00% in these groups, respectively. The positive rates of T SPOT assay were significantly higher than those of PPD skin test in all patients, in those with CD4 < 200/μl and with anti-tuberculosis treatment < 2 months ( P < 0.05 ). Conclusion T SPOT assay was more sensitive than conventional PPD skin test, and can be applied in the diagnosis of MTB infections for AIDS patients.
- [1]崔为国,王林,王哲,等.HIV/AIDS患者结核菌素反应及与CD4细胞关系[J].2005,(6).
- [2]苏振修,甄新安,王国斌,等.ESAT6抗原刺激产生INFγ水平测定在HIV感染者中对结核病诊断的价值[J].2007,(9).
- [3]蒋卫民,潘孝彰,康来仪.CD4淋巴细胞计数在HIV感染中的地位和重要性[J].2001,(6).
- [4]Lalvani A,Alvarez L,Bryant G,etc.Dynamic antigen-specific T-cell responses after point-source exposure to Mycobacterium tuberculosis.[J].2006,174(7).
- [5]Liebeschuetz S,Deeks J,Pathan AA,etc.Diagnosis of tuberculosis in South African children with a T-cell-based assay: a prospective cohort study.[J].2004,364(9452).
- [6]质检出版社.HIV/AIDS诊断标准及处理原则[S].1995.
- [7]Ann Chapman,Mwansa Munkanta,Katalin Wilkinson,etc.Rapid detection of active and latent tuberculosis infection in HIV-positive individuals by enumeration ofMycobacterium tuberculosis-specific T cells[J].2002,16(17).
- [8]Furrer H,Fierz W,Vernazza P,etc.Conventional tuberculin skin testing versus T-cell-based assays in the diagnosis of latent tuberculosis infection in HIV-positive patients.[J].2007,21(3).
- [9]Balandrano Campos-S,Garcia Garcia-ML,Valdespino Gomez-JL,etc.Underestimation of Mycobacterium tuberculosis infection in HIV-infected subjects using reactivity to tuberculin and anergy panel.[J].2000,29(2).