Preparation and initial identification of monoclonal antibody of anti-vibrio fluvialis

( views:1177, downloads:0 )
FU Kai-fei(Clinical Laboratory, Navy General Hospital, Beijing 100048, China)
MA Cong(Clinical Laboratory, Navy General Hospital, Beijing 100048, China)
GUO Jian-wei(Clinical Laboratory, Navy General Hospital, Beijing 100048, China)
HAO Xiu-hong(Clinical Laboratory, Navy General Hospital, Beijing 100048, China)
Journal Title:
Chinese Journal of Nautical Medicine and Hyperbaric Medicine
Volume 19, Issue 04, 2012
Key Word:
Vibrio fluvialis;Monoclonal antibody;ELISA

Abstract: Objective To prepare high-performance and specific monoclonal antibody of vibrio fluvialis,and to provide a technical platform for the development of a rapid ELISA kit.Methods BALB/c mice (8 weeks) were immunized with inactivated vibrio fluvialis,and hybridoma cell strains of anti-vibrio fluvialis were prepared with cell-fusion technology.Chromatosomes of hybridoma cells were identified with Giemsa staining,and the potency and cross-reactivity of anti-vibrio fluvialis as well as other important marine bacteria were screened and identified with ELISA.Results Four strains of hybridoma (named as 01H10,02F12,05F3 and 03G10 ) were obtained,all having the features of hybridoma cell chromatosomes,and with good specificity and immunorcactivity.The hybridoma also had favourable specificity and immunoreactivity.Conclusions Specific antibodies of anti-vibriofluvialis prepared and screened in the study would be useful for the development of the rapid detection kit of vibrio fluvialis.

  • [1]Etinosa O,Igbinosa,Anthony 1,et al.Vibrio fluvialis:an unusual enteric pathogen of increasing public health concern.Int J Environ Res Public Health,2010,7(10):3628-3643.
  • [2]Huang KC,Hsu RW.Vibrio fluvialis hemorrhagic cellulitis and cerebritis.Clin Infect Dis,2005,40(9):e75-77.
  • [3]Lai CH,Hwang CK,Chin C,et al.Severe watery diarrhoea and bacteraemia caused by Vibrio fluvialis.J Infect,2006,52(3):e95-98.
  • [4]Rahim Z.Enttertoxigenicity of Vibrio fluvialis strains isolated from fresh water enviorenment.J Diarhoeal Dis Res,1994,12(4):290-291.
  • [5]Chikahira M,Hamada K.Enterotoxigenic substance and other toxins produced by Vibrio fluvialis and Vibrio furnissii.Nippon Juigaku Zasshi,1988,50(4):865-873.
  • [6]Alam M,Miyoshi S,Shinoda S.Production of antigenically related exocellular elastolytic proteases mediating hemagglutination by vibrios.Microbiol Immunol,1995,39(1):67-70.
  • [7]李太武,苏秀榕,丁明进.皱纹盘鲍脓疱病菌——河流弧菌Ⅱ胞外产物的研究.辽宁师范大学学报:自然科学版,1999,22(3):231-235.
  • [8]Lee SK,Wang HZ,Law SH,et al.Analysis of the 16S-23SrDNA intergenic spacers(IGSs)of marine vibrios for species specific signature DNA sequences.Mar Pollut Bull,2002,44(5):412-420.
  • [9]曹际娟,赵昕,孙哲平,等.PCR结合变性高效液相色谱快速检测水产品中河流弧菌.中国卫生检验杂志,2008,18(11):2187-2189.
  • [10]Crisafi F,Denaro R,Genovese M,et al.Comparison of 16SrDNA and toxR genes as targets for detection of Vibrio anguillarum in Dicentrarchus labrax kidney and liver.Res Microbiol,2011,162(3):223-230.
  • [11]Buzina W,Feierl G,Haas D,et al.Lethal brain abscess due to the fungus Scedosporium apiospermum(teleomorph Pseudallescheria boydii)after a near-drowning incident:case report and review of the literature.Med Mycol,2006,44(5):473-477.
  • [12]Nhung PH,Ohkusu K,Miyasaka J,et al.Rapid and specific identification of 5 human pathogenic Vibrio species by multiplex polymerase chain reaction targeted to DNA.J Gene Diagn Microbiol Infect Dis,2007,59(3):271-275.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615