Effect of hyperbaric oxygen on HMGB1 expression in the rat model of inflammatory responses after spinal cord injury

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YANG Jing(Department of Hyperbaric Oxygen,Beijing Chaoyang Hospital, Capital Medical University,Beijing 100020, China)
WANG Guo-zhong()
LIU Xue-hua(Department of Hyperbaric Oxygen,Beijing Chaoyang Hospital, Capital Medical University,Beijing 100020, China)
WANG Zhi-wei()
GAO Chun-jin(Department of Hyperbaric Oxygen,Beijing Chaoyang Hospital, Capital Medical University,Beijing 100020, China)
SU Qing-jun()
Journal Title:
Chinese Journal of Nautical Medicine and Hyperbaric Medicine
Volume 19, Issue 04, 2012
Key Word:
Hyperbaric oxygen;Spinal cord injury;High mobility group protein B1

Abstract: Objective To investigate the effect of hyperbaric oxygen on high mobility group protein B1 (HMGB1) expression in the tissues of spinal cord injury,and the characteristic of secondary inflammatory response after spinal cord injury.Methods According to the randomization table,160 healthy and adult Sprague-Dawley (SD) rats were equally divided into 4 groups,including the spinal cord injury group,the spinal cord injury + hyperbaric oxygen group,the sham operation group and the sham operation + hyperbaric oxygen group.Each group was further randomly and equally divided into 4 subgroups:the 1st,3rd,7th,14th day groups.BBB score was used to evaluate functional rehabilitation of indlimb and the methods of PCR,Western blot,immunohistochemistry were performmed to determine the expressions of HMGB1 and nuclear factor kappa B (NF-κB) in the damaged tissues at different time points after spinal cord injury.Results The levels of mRNA and the expressions of protein of HMGB1 and NF-κB in the damaged tissues after spinal cord injury were significantly increased (P <0.01 ).By pre and posthyperbaric oxygen intervention,No significant differences the level of HMGB1 mRNA and the expression of HMGB1 protein after spinal cord injury (P >0.05) on the 1st and 3rd days,but there were significant differences on the 7th and 14th days (P < 0.05 ).Hyperbaric oxygen intervention reduced the level of NF-κB mRNA and NF-κB protein expression,without significant difference (P>0.05) on the 1st day,with significant differences (P <0.05) on the 3rd,7th and 14th days.After hyperbaric oxygen intervention,the BBB scores were significantly higher on the 7 th and 14th days (P < 0.05).Conclusions Hyperbaric oxygen can reduce secondary inflammatory response after spinal cord injury by restricting the expression of HMGB1 and promoting the repair of neurological function.

  • [1]Donnelly DJ,Popovich PG.Inflammation and its role in neuroprotection,axonal regeneration and functional recovery after spinal cord injury.Exp Neurol,2008,209(2):378-388.
  • [2]Stahel PF,Flierl MA.Targted modulation of the neuroinflammatory response after spinal cord injury:the engoing quest for the "holy grail".Am J Pathol,2010,177(6):2685-2687.
  • [3]Zhou M,Wu G,Wu LJ.Neuronal and microglial mechanisms of neuropathic pain.Mol Brain,2011,4:31.
  • [4]Alexander JK,Popovich PG.Neuroinflammation in spinal cord injury:therapeutic targets for neuroprotection and regeneration.Prog Brain Res,2009,175:125-137.
  • [5]Kwon BK,Fisher CG,Dvorak MF,et al.Strategies to promote neural repair and regeneration after spinal cord injury.Spine,2005,30(17 Supp 1):S3-S13.
  • [6]Li SH,Guo PD,Wang WJ.Current situation and progression in the treatment of spinal cord injury.中 国骨伤,2010,23(1):70-73.
  • [7]高春锦,杨捷云,翟晓辉.高压氧医学基础与临床.北京:人民卫生出版社,2008:120.
  • [8]李宁.高压氧与脊髓损伤.重庆医学,2006,35(19):1729-1730.
  • [9]Al-Waili NS,Butler GJ,Beale J,et al.Hyperbaric oxygen in the treatment of patients with cerebral stroke,brain trauma,and neurologic disease.Adv Ther,2005,22(6):659-678.
  • [10]Tai PA,Chang CK,Niu KC,et al.Attenuating experimental spinal cord injury by hyperbaric oxygen:stimulating production of vasculoendothelial and glial cell line-derived neurotrophic growth factors and interleukin-10.J Neurotrauma,2010,27(6):1121-1127.
  • [11]张君亮,龙绍华,徐艳,等.高压氧辅助治疗不完全性脊髓损伤的疗效观察.江西医药,2011,46(8):730-732.
  • [12]田洪成,金永成.高压氧对脊髓损伤患者的辅助治疗作用研究.中国现代医药杂志,2011,13(9):10-12.
  • [13]王国忠,杨晶,苏庆军,等.高压氧对脊髓损伤患者术后血清肿瘤坏死因子-α及白细胞介素-6水平的影响.中华航海医学与高气压医学杂志,2012,19(2):102-104.
  • [14]Wang H,Li W,Goldstein R,et al.HMGB1 as a potential therapeutic target.Novartis Found Symp,2007,280:73-85.
  • [15]Yang H,Wang H,Czura CJ,et al.The cytokine activity of HMGB1.J Leukocyte Biol,2005,78(1):1-8.
  • [16]Beijnum JR,Buurman WA,Griffioen AW.Convergence and amplification of toll-like receptor(TLR)and receptor for advanced glycation end products(RAGE)signaling pathways via high mobility group B1(HMGB1).Angiogenesis,2008,11(1):91-99.
  • [17]Maede N.Experimental studies on the effect of decompress prccedures and hyperbaric oxygenation for the treatment of spinal cord injury.J Nara Med Assoc,1965,16:429-447.
  • [18]Holbach KH,Wassmann H,Linke D.The use of hyperbaric oxygenation in the treatment of spinal cord lesions.Eur Neurol,1977,16(1-6):213-221.
  • [19]胥正泉,孙永明,王海斌.高压氧对大鼠脊髓损伤后炎症因子的影响.中国现代医药杂志,2009,11(10):17-19.
  • [20]Kawabata H,Setoguchi T,Yone K,et al.High mobility group box 1 is upregulated after spinal cord injury and is associated with neuronal cell apoptosis.Spine(Phila Pa1976),2010,35(11):1109-1115.
  • [21]欧阳军,彭心宇,李锋.晚期炎症介质-高迁移率族蛋白B-1现代生物医学进展,2010,10(5):968-971.
  • [22]Li JM,Quan ZX,Liu B.Research progress of the NF-κB signaling pathway on acute secondary response to spinal cord injury.J Traum Surg,2009,11(1):85-87.
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